Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605163340_vk4r AGTAAAAGCAGTCACTTCACTGGGCATTGCTG(+CAGTGT)GTACCGGCAGGGGGTGTGTAGGTTAGTTCTG NGG 10 8 17 8 24 0 100 5 10
    240605163338_03yp TGTGTCAGAACTAACCTACACACCCCCTGCCG(+CAGTGT)GTACCAGCAATGCCCAGTGAAGTGACTGCTT NGG 10 8 17 8 24 0 100 5 10
    240605163336_ard5 CTCCGTTACGATATATCCTAGCCACATGCAGG(+CAGTGT)GTACCACATAGGATTTTCTATATTGAAAGAA NGG 10 8 17 8 24 0 100 5 10
    240605163335_acjj AAGTATTCTTTCAATATAGAAAATCCTATGTG(+CAGTGT)GTACCCTGCATGTGGCTAGGATATATCGTAA NGG 10 8 17 8 24 0 100 5 10
    240605163334_69n0 CCTGGGCCTGTTGGAGTGTAGCTATGTTGGTG(+CAGTGT)GATCCTGTATACAGGTGAAAAAGTGCCATTG NGG 10 8 17 8 24 0 100 5 10
    240605163332_ytij AACGACAATGGCACTTTTTCACCTGTATACAG(+CAGTGT)GATCCACCAACATAGCTACACTCCAACAGGC NGG 10 8 17 8 24 0 100 5 10
    240605163331_ifee AAATCAGGAATCTGACTCTTGCTAGGCTACCG(+CAGTGT)GATCCCTGCATGCTCCTCTGAGTTTGGGCAG NGG 10 8 17 8 24 0 100 5 10
    240605163329_rag7 TCATGCTGCCCAAACTCAGAGGAGCATGCAGG(+CAGTGT)GATCCGGTAGCCTAGCAAGAGTCAGATTCCT NGG 10 8 17 8 24 0 100 5 10
    240605163327_i8sy CAAAAAATATCTGACAATAAAATAGCCCACTG(+CAGTGT)GATCCTGTTCAGGCTGGCTGATATCTTGATT NGG 10 8 17 8 24 0 100 5 10
    240605163325_9mew TGTTGAATCAAGATATCAGCCAGCCTGAACAG(+CAGTGT)GATCCAGTGGGCTATTTTATTGTCAGATATT NGG 10 8 17 8 24 0 100 5 10