Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605163251_uflr CATCTCTATAACCGTTTGCTCTTGGACTCCCG(+CAGTGT)GATCCCTGTACCCCAGGTTGTTTTCCCTGTA NGG 10 8 17 8 24 0 100 5 10
    240605163250_qkkg CAGGTTACAGGGAAAACAACCTGGGGTACAGG(+CAGTGT)GATCCGGGAGTCCAAGAGCAAACGGTTATAG NGG 10 8 17 8 24 0 100 5 10
    240605163247_i8er CATCTCTATAACCGTTTGCTCTTGGACTCCCG(+CAGTGT)GATCCCTGTACCCCAGGTTGTTTTCCCTGTA NGG 10 8 17 8 24 0 100 5 10
    240605163246_81w8 CAGGTTACAGGGAAAACAACCTGGGGTACAGG(+CAGTGT)GATCCGGGAGTCCAAGAGCAAACGGTTATAG NGG 10 8 17 8 24 0 100 5 10
    240605163244_h8w8 ATGAGCAAAGGCAGGGACCGGGGCTTGCTAGG(+CAGTGT)GTACCAGCCCAGTTCTCCAGCCGTGAAGTGG NGG 10 8 17 8 24 0 100 5 10
    240605163243_oka8 GAGACCCACTTCACGGCTGGAGAACTGGGCTG(+CAGTGT)GTACCCTAGCAAGCCCCGGTCCCTGCCTTTG NGG 10 8 17 8 24 0 100 5 10
    240605163240_xgf5 CCCCTGCCACCCAACCAGGTCTTTGGTGGGGG(+CAGTGT)GATCCCAAAGCCATTCTGGAAAGGGCTCCAG NGG 10 8 17 8 24 0 100 5 10
    240605163238_mfa1 TTCCTCTGGAGCCCTTTCCAGAATGGCTTTGG(+CAGTGT)GATCCCCCCACCAAAGACCTGGTTGGGTGGC NGG 10 8 17 8 24 0 100 5 10
    240605163237_ez6v TCCTGTGCCTGCCTTCTCTTTCTGCCTGTATG(+CAGTGT)GATCCCCAAAGGCAGTGAGTTCCTGGCTTGT NGG 10 8 17 8 24 0 100 5 10
    240605163235_5x0h AAAGGACAAGCCAGGAACTCACTGCCTTTGGG(+CAGTGT)GATCCATACAGGCAGAAAGAGAAGGCAGGCA NGG 10 8 17 8 24 0 100 5 10