Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605163141_nyv9 CATGATTTAATGTTGACACCTTGGTCTAGAAG(+CAGTGT)GATCCACTCGGGGTTGCTAGGCTCCCTTACA NGG 10 8 17 8 24 0 100 5 10
    240605163140_mpbc CAAGCTGTAAGGGAGCCTAGCAACCCCGAGTG(+CAGTGT)GATCCTTCTAGACCAAGGTGTCAACATTAAA NGG 10 8 17 8 24 0 100 5 10
    240605163138_3jlf GGTGTCATGGTCACCTCATAGGTGGAGGGTGG(+CAGTGT)GTACCTAAAACAAGGACAGAAGCCAGCAGCT NGG 10 8 17 8 24 0 100 5 10
    240605163137_vuai ACACTAGCTGCTGGCTTCTGTCCTTGTTTTAG(+CAGTGT)GTACCCACCCTCCACCTATGAGGTGACCATG NGG 10 8 17 8 24 0 100 5 10
    240605163136_0shl AAGAACCTGGCTTTTGTCCTTTTGACTCTATG(+CAGTGT)GTACCACATTCTGTCAGTTCACCATGTGACA NGG 10 8 17 8 24 0 100 5 10
    240605163134_lz6d AGCCATGTCACATGGTGAACTGACAGAATGTG(+CAGTGT)GTACCATAGAGTCAAAAGGACAAAAGCCAGG NGG 10 8 17 8 24 0 100 5 10
    240605163132_7lmc ACCATGCGGTGGGCGAAGACGATTGTCTGGGG(+CAGTGT)GATCCAGGGCCATGGCTGGGAGAAGTCAAGG NGG 10 8 17 8 24 0 100 5 10
    240605163130_17rd CTTTACCTTGACTTCTCCCAGCCATGGCCCTG(+CAGTGT)GATCCCCCAGACAATCGTCTTCGCCCACCGC NGG 10 8 17 8 24 0 100 5 10
    240605163128_yj3r GGGGAGTCAGAATGGGAAAGATGCATACGCTG(+CAGTGT)GATCCTTCAGAAATTTACAGGGCAGGGCTCA NGG 10 8 17 8 24 0 100 5 10
    240605163127_e9mt AACACTGAGCCCTGCCCTGTAAATTTCTGAAG(+CAGTGT)GATCCAGCGTATGCATCTTTCCCATTCTGAC NGG 10 8 17 8 24 0 100 5 10