Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605163214_66f8 AAGCTTCCAAGTGCTGGGGAAAGTCCAGCTAG(+CAGTGT)GATCCCATTGGTTCCTTGGCTTGATGTGCAA NGG 10 8 17 8 24 0 100 5 10
    240605163212_b9bl TCTGTTTGCACATCAAGCCAAGGAACCAATGG(+CAGTGT)GATCCTAGCTGGACTTTCCCCAGCACTTGGA NGG 10 8 17 8 24 0 100 5 10
    240605163211_3dcm AGTGTGTGTTCCTCCTGGAAGTAGAAAGAGAG(+CAGTGT)GATCCGGGACAAATCCAGACAAGTCCAGGGG NGG 10 8 17 8 24 0 100 5 10
    240605163209_0kcj ACATACCCCTGGACTTGTCTGGATTTGTCCCG(+CAGTGT)GATCCTCTCTTTCTACTTCCAGGAGGAACAC NGG 10 8 17 8 24 0 100 5 10
    240605163207_o3ou GCTTGACAGTGTCACCCAGTGGTGACACTAGG(+CAGTGT)GATCCTGGTACACAGGGCACACCTTTCACTG NGG 10 8 17 8 24 0 100 5 10
    240605163205_6u63 CCTCACAGTGAAAGGTGTGCCCTGTGTACCAG(+CAGTGT)GATCCCTAGTGTCACCACTGGGTGACACTGT NGG 10 8 17 8 24 0 100 5 10
    240605163204_7fbf TAGAAACATAAAACCAGATACATGCAAATGAG(+CAGTGT)GATCCTAACTATATTCTCAGCCACAGAGGCT NGG 10 8 17 8 24 0 100 5 10
    240605163202_dg8x AGAAGAGCCTCTGTGGCTGAGAATATAGTTAG(+CAGTGT)GATCCTCATTTGCATGTATCTGGTTTTATGT NGG 10 8 17 8 24 0 100 5 10
    240605163200_xb2a TGGCCCAGCGTATGCACTGGCACTGGGAAGGG(+CAGTGT)GATCCAGAAGCGGTAGCTGAAGGAGAGACTG NGG 10 8 17 8 24 0 100 5 10
    240605163159_681n CGGGACAGTCTCTCCTTCAGCTACCGCTTCTG(+CAGTGT)GATCCCCTTCCCAGTGCCAGTGCATACGCTG NGG 10 8 17 8 24 0 100 5 10