Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605163157_hs9j GTGACGCTGGGTGCTGGAAGTAGCAGCTGAGG(+CAGTGT)GATCCTTCTTGGTCCTCAGGATTCTGGGAAT NGG 10 8 17 8 24 0 100 5 10
    240605163155_f6w0 TTCAAATTCCCAGAATCCTGAGGACCAAGAAG(+CAGTGT)GATCCCTCAGCTGCTACTTCCAGCACCCAGC NGG 10 8 17 8 24 0 100 5 10
    240605163153_jwab TCAAGGACGCAACCTGGGGTTGCCAAGACCAG(+CAGTGT)GATCCAGACCCACGAGTGATGGGCCGAGGCA NGG 10 8 17 8 24 0 100 5 10
    240605163152_yji0 TGTTGTGCCTCGGCCCATCACTCGTGGGTCTG(+CAGTGT)GATCCTGGTCTTGGCAACCCCAGGTTGCGTC NGG 10 8 17 8 24 0 100 5 10
    240605163150_reyi AGTGTAGCATGCGGTCGGCATTGAGAACCTTG(+CAGTGT)GTACCAAAGGCCCCTGAGTCTTCCACTTACT NGG 10 8 17 8 24 0 100 5 10
    240605163148_0qh4 AAGAGAGTAAGTGGAAGACTCAGGGGCCTTTG(+CAGTGT)GTACCAAGGTTCTCAATGCCGACCGCATGCT NGG 10 8 17 8 24 0 100 5 10
    240605163147_wkum TACACACTAATCAGTTTTGTGCTGACAGAAAG(+CAGTGT)GATCCTAGTTAAAATACTGATTCTCAAAAGT NGG 10 8 17 8 24 0 100 5 10
    240605163146_m32z ATGTTACTTTTGAGAATCAGTATTTTAACTAG(+CAGTGT)GATCCTTTCTGTCAGCACAAAACTGATTAGT NGG 10 8 17 8 24 0 100 5 10
    240605163144_6alp ACCCTGAGATCCTTGTGTGAGCAAGATCCTGG(+CAGTGT)GATCCTCTGATCCTCTGATCCTGGGCGTGTT NGG 10 8 17 8 24 0 100 5 10
    240605163142_n9ww ACTCTAACACGCCCAGGATCAGAGGATCAGAG(+CAGTGT)GATCCCAGGATCTTGCTCACACAAGGATCTC NGG 10 8 17 8 24 0 100 5 10