Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605163022_xxv2 CCTGATGCTACAGAAGCCAGATGAGGGGACTG(+CAGTGT)GATCCCCTGGAACTGGAGGTACAGATGGCTG NGG 10 8 17 8 24 0 100 5 10
    240605163019_ldev GCTCACAGCCATCTGTACCTCCAGTTCCAGGG(+CAGTGT)GATCCAGTCCCCTCATCTGGCTTCTGTAGCA NGG 10 8 17 8 24 0 100 5 10
    240605163017_1p71 GCCACGCCTCGCTCTCTGTGTACAGCAATGGG(+CAGTGT)GATCCAGGAAGCAGGATGGGCCAGGAACCCG NGG 10 8 17 8 24 0 100 5 10
    240605163015_ct6a GCCTTCGGGTTCCTGGCCCATCCTGCTTCCTG(+CAGTGT)GATCCCCATTGCTGTACACAGAGAGCGAGGC NGG 10 8 17 8 24 0 100 5 10
    240605163014_23mr AGACTATGTGCTACCTTCACTTGCTAATGCAG(+CAGTGT)GATCCAAAGAATGCAGGCAAACCCTACAAAT NGG 10 8 17 8 24 0 100 5 10
    240605163013_hqgf GCTACATTTGTAGGGTTTGCCTGCATTCTTTG(+CAGTGT)GATCCTGCATTAGCAAGTGAAGGTAGCACAT NGG 10 8 17 8 24 0 100 5 10
    240605163011_duf4 TAAATGGGACAAAGACATAGGCAAAAAACATG(+CAGTGT)GATCCTAAGTAGAATCAGATATTTATCTCTT NGG 10 8 17 8 24 0 100 5 10
    240605163010_d0su AGAAAAAGAGATAAATATCTGATTCTACTTAG(+CAGTGT)GATCCATGTTTTTTGCCTATGTCTTTGTCCC NGG 10 8 17 8 24 0 100 5 10
    240605163008_0gbc ATACTTTCCACATCTGGGGAATGTAAGCTAGG(+CAGTGT)GTACCTCATCTGGGATTTATCTTATTCTTCC NGG 10 8 17 8 24 0 100 5 10
    240605163007_52a4 GATCTGGAAGAATAAGATAAATCCCAGATGAG(+CAGTGT)GTACCCTAGCTTACATTCCCCAGATGTGGAA NGG 10 8 17 8 24 0 100 5 10