Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605162915_zxa9 CTGGGGCTTCTCTCCACTTTCTGGCAAGGAAG(+CAGTGT)GTACCAGGGTCACAGACTCCCACACAGTCTC NGG 10 8 17 8 24 0 100 5 10
    240605162913_rvxm TACTTGAGACTGTGTGGGAGTCTGTGACCCTG(+CAGTGT)GTACCTTCCTTGCCAGAAAGTGGAGAGAAGC NGG 10 8 17 8 24 0 100 5 10
    240605162911_85gn CCTGTCAGTGAGGGGGGTTGACTCCTGTGTGG(+CAGTGT)GTACCATTGGGGAAGGATGGATTTATCACAC NGG 10 8 17 8 24 0 100 5 10
    240605162909_zc6y GGAGAGTGTGATAAATCCATCCTTCCCCAATG(+CAGTGT)GTACCCACACAGGAGTCAACCCCCCTCACTG NGG 10 8 17 8 24 0 100 5 10
    240605162908_f094 AGACCAAGAACAGCAGAAGCTCACAGAGTGAG(+CAGTGT)GATCCAGGGAAGCCTTGGCAGGCTGTGGGCA NGG 10 8 17 8 24 0 100 5 10
    240605162905_1rxi CCGCTTGCCCACAGCCTGCCAAGGCTTCCCTG(+CAGTGT)GATCCTCACTCTGTGAGCTTCTGCTGTTCTT NGG 10 8 17 8 24 0 100 5 10
    240605162903_hubn CACCTGGGAACCACTCGACTACTGACCCAAAG(+CAGTGT)GTACCCTCCCCAGGGCTGCTCAGGACCAGTG NGG 10 8 17 8 24 0 100 5 10
    240605162901_9cug CTGCACACTGGTCCTGAGCAGCCCTGGGGAGG(+CAGTGT)GTACCTTTGGGTCAGTAGTCGAGTGGTTCCC NGG 10 8 17 8 24 0 100 5 10
    240605162859_jd78 CACCTGGGAACCACTCGACTACTGACCCAAAG(+CAGTGT)GTACCCTCCCCAGGGCTGCTCAGGACCAGTG NGG 10 8 17 8 24 0 100 5 10
    240605162858_xwdv CTGCACACTGGTCCTGAGCAGCCCTGGGGAGG(+CAGTGT)GTACCTTTGGGTCAGTAGTCGAGTGGTTCCC NGG 10 8 17 8 24 0 100 5 10