Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605162809_eelv CTCCATGATGGTGGGACACCTGCTCCCGTGTG(+CAGTGT)GATCCTATAGCTAGTGTCCTTCAGTCCACTG NGG 10 8 17 8 24 0 100 5 10
    240605162807_ldq5 GCACGCAGTGGACTGAAGGACACTAGCTATAG(+CAGTGT)GATCCACACGGGAGCAGGTGTCCCACCATCA NGG 10 8 17 8 24 0 100 5 10
    240605162806_3khm GGGTTTTTCTAGTCAGTGAGATACTCCAACTG(+CAGTGT)GATCCCAAAGATGGAGTACCTTAGCGTCACT NGG 10 8 17 8 24 0 100 5 10
    240605162804_ub3w AGTTCAGTGACGCTAAGGTACTCCATCTTTGG(+CAGTGT)GATCCAGTTGGAGTATCTCACTGACTAGAAA NGG 10 8 17 8 24 0 100 5 10
    240605162803_0c55 TGGTGGGAGTTACGGCTGCTGGTGGGAGTCTG(+CAGTGT)GTACCTTTCTGGTTGAGCAATGCAGCAAAAT NGG 10 8 17 8 24 0 100 5 10
    240605162802_b2or GTGGAATTTTGCTGCATTGCTCAACCAGAAAG(+CAGTGT)GTACCAGACTCCCACCAGCAGCCGTAACTCC NGG 10 8 17 8 24 0 100 5 10
    240605162800_o82h GATGCAGGGCTGGAAGGAGCCAAGCAAGGAGG(+CAGTGT)GTACCCAGGAGGAGCTGGCAGGGCGAGAACA NGG 10 8 17 8 24 0 100 5 10
    240605162758_lsqo CCAGGTGTTCTCGCCCTGCCAGCTCCTCCTGG(+CAGTGT)GTACCCTCCTTGCTTGGCTCCTTCCAGCCCT NGG 10 8 17 8 24 0 100 5 10
    240605162757_8w5f CTCTACTCTTTCTTGCCATCGCCCCTGGCATG(+CAGTGT)GATCCAGAGACTCAGGCTACAACAGGAAGCC NGG 10 8 17 8 24 0 100 5 10
    240605162755_prvd AAAGTGGCTTCCTGTTGTAGCCTGAGTCTCTG(+CAGTGT)GATCCATGCCAGGGGCGATGGCAAGAAAGAG NGG 10 8 17 8 24 0 100 5 10