Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605162840_wmpl GGCCTGGCTTTCGCAGGAGGGTCTGGCAGCTG(+CAGTGT)GATCCATGTCTGTCCACCCACGCGGGGGAAC NGG 10 8 17 8 24 0 100 5 10
    240605162838_e9bm AGGAAGTTCCCCCGCGTGGGTGGACAGACATG(+CAGTGT)GATCCAGCTGCCAGACCCTCCTGCGAAAGCC NGG 10 8 17 8 24 0 100 5 10
    240605162837_csq3 GGGACCATCTCCTGAAGCCTCAGCACTGCCAG(+CAGTGT)GATCCACATTCCTGACCTGGATACTGGCTGA NGG 10 8 17 8 24 0 100 5 10
    240605162835_cazm ACTTTTCAGCCAGTATCCAGGTCAGGAATGTG(+CAGTGT)GATCCTGGCAGTGCTGAGGCTTCAGGAGATG NGG 10 8 17 8 24 0 100 5 10
    240605162834_y9x8 TGTAGCCTTGCCCTGTCTTGGGCCTGGTGGAG(+CAGTGT)GATCCTGGAGCCTCTTGGTGGTGCATTGTCC NGG 10 8 17 8 24 0 100 5 10
    240605162832_8u1c AGAAAGGACAATGCACCACCAAGAGGCTCCAG(+CAGTGT)GATCCTCCACCAGGCCCAAGACAGGGCAAGG NGG 10 8 17 8 24 0 100 5 10
    240605162829_u0cq ATTTGGCAACCTAACCAGGCAGCTGCCCCTGG(+CAGTGT)GATCCCTAGCTTCTCCAACCTGAGCACCAGC NGG 10 8 17 8 24 0 100 5 10
    240605162828_drbf GGAATGCTGGTGCTCAGGTTGGAGAAGCTAGG(+CAGTGT)GATCCCAGGGGCAGCTGCCTGGTTAGGTTGC NGG 10 8 17 8 24 0 100 5 10
    240605162827_rbdf GAGCCCTTTATTGGCTTGGATTAGCTGTGTAG(+CAGTGT)GATCCAGGGACTGTCCTCGTCCTCGGGTTGG NGG 10 8 17 8 24 0 100 5 10
    240605162824_9y4c GCAAGCCAACCCGAGGACGAGGACAGTCCCTG(+CAGTGT)GATCCTACACAGCTAATCCAAGCCAATAAAG NGG 10 8 17 8 24 0 100 5 10