Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605162822_pndi AATGTCTCCTCTCCAGTAGCTCCACAGAGGTG(+CAGTGT)GTACCTCAGCCAAAGCCATTCCTAACCCATG NGG 10 8 17 8 24 0 100 5 10
    240605162821_fup5 AGGTTCATGGGTTAGGAATGGCTTTGGCTGAG(+CAGTGT)GTACCACCTCTGTGGAGCTACTGGAGAGGAG NGG 10 8 17 8 24 0 100 5 10
    240605162820_6q6m AAAAAGCTTTATTAAAAGTTAGCCCTTACAAG(+CAGTGT)GATCCTATTAAAATATACACATTTTAAATAA NGG 10 8 17 8 24 0 100 5 10
    240605162819_3od9 TGCATTTATTTAAAATGTGTATATTTTAATAG(+CAGTGT)GATCCTTGTAAGGGCTAACTTTTAATAAAGC NGG 10 8 17 8 24 0 100 5 10
    240605162818_1l3z TCTAATCTCTGTCAAAAGGGAGGTTAGGAAAG(+CAGTGT)GTACCCCCTCCCTTGTAAACACTGAAATACA NGG 10 8 17 8 24 0 100 5 10
    240605162815_1hbg AGATCTGTATTTCAGTGTTTACAAGGGAGGGG(+CAGTGT)GTACCTTTCCTAACCTCCCTTTTGACAGAGA NGG 10 8 17 8 24 0 100 5 10
    240605162814_wdo0 CTGCCAGAGGCTTGCCCAAGAAAAGGAGTCGG(+CAGTGT)GTACCTGTCAGATGCTACCTGGAGGGTCAGG NGG 10 8 17 8 24 0 100 5 10
    240605162812_43z7 GATGACCTGACCCTCCAGGTAGCATCTGACAG(+CAGTGT)GTACCCGACTCCTTTTCTTGGGCAAGCCTCT NGG 10 8 17 8 24 0 100 5 10
    240605162811_hh9v AGGTTGGGATGTCCCTGTTGCTAGCCCATTAG(+CAGTGT)GATCCTAGCTCCAATTCTGACCTTGAAAAAG NGG 10 8 17 8 24 0 100 5 10
    240605162810_goso CTTGACTTTTTCAAGGTCAGAATTGGAGCTAG(+CAGTGT)GATCCTAATGGGCTAGCAACAGGGACATCCC NGG 10 8 17 8 24 0 100 5 10