Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605162723_jxfd AGACCAAGTTTCTCACTAACCAGGTACAAAAG(+CAGTGT)GATCCAACCAACGTATGAGTTACGGTGTTTG NGG 10 8 17 8 24 0 100 5 10
    240605162721_t7dy AGAAACAAACACCGTAACTCATACGTTGGTTG(+CAGTGT)GATCCTTTTGTACCTGGTTAGTGAGAAACTT NGG 10 8 17 8 24 0 100 5 10
    240605162720_ck1n TTCTATTAAAACCTGTCCCTGGTACTATCATG(+CAGTGT)GTACCAAGCTGTGTAAAACACTGCACTGAAT NGG 10 8 17 8 24 0 100 5 10
    240605162719_lyb2 AACAAATTCAGTGCAGTGTTTTACACAGCTTG(+CAGTGT)GTACCATGATAGTACCAGGGACAGGTTTTAA NGG 10 8 17 8 24 0 100 5 10
    240605162717_2fq8 AGGGGTTACCAGTATTCGTTGCTCTTGCAGAG(+CAGTGT)GATCCAGGTTTGCTCCTAGCACTCACACAGT NGG 10 8 17 8 24 0 100 5 10
    240605162715_hnhl GAGCTACTGTGTGAGTGCTAGGAGCAAACCTG(+CAGTGT)GATCCTCTGCAAGAGCAACGAATACTGGTAA NGG 10 8 17 8 24 0 100 5 10
    240605162714_ga9k TACAGTTGTGCAACTAAGTCTGGTTGTGCCTG(+CAGTGT)GATCCAACTGCTACCAGCATTAAAAACACTT NGG 10 8 17 8 24 0 100 5 10
    240605162712_jafs AACCCAAGTGTTTTTAATGCTGGTAGCAGTTG(+CAGTGT)GATCCAGGCACAACCAGACTTAGTTGCACAA NGG 10 8 17 8 24 0 100 5 10
    240605162711_w38m AAACCCTTTGTTCCTGCTGACTCCACCCACAG(+CAGTGT)GATCCATGCAGATCTCACAGAATGCTGAGAT NGG 10 8 17 8 24 0 100 5 10
    240605162709_n2hy GAAGCATCTCAGCATTCTGTGAGATCTGCATG(+CAGTGT)GATCCTGTGGGTGGAGTCAGCAGGAACAAAG NGG 10 8 17 8 24 0 100 5 10