Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605162652_1d46 CCTTAGGCAGTGCCTGCTACTCCAAAGGGGAG(+CAGTGT)GTACCGTTAGTGTGCAGGAATGAGACACTTG NGG 10 8 17 8 24 0 100 5 10
    240605162650_v2jb CCTTCCAAGTGTCTCATTCCTGCACACTAACG(+CAGTGT)GTACCTCCCCTTTGGAGTAGCAGGCACTGCC NGG 10 8 17 8 24 0 100 5 10
    240605162649_0bpg GTGCAGGAATGAGACACTTGGAAGGCAGATGG(+CAGTGT)GATCCAAGAGAAAGGCATACTTGGTAAACTA NGG 10 8 17 8 24 0 100 5 10
    240605162647_m51d CAATATAGTTTACCAAGTATGCCTTTCTCTTG(+CAGTGT)GATCCCATCTGCCTTCCAAGTGTCTCATTCC NGG 10 8 17 8 24 0 100 5 10
    240605162646_bx3h CATCTACTTTGCCTTTTTGTGCAGGTTATATG(+CAGTGT)GATCCTGCACCGCTCATTACAAAGGTCTGCA NGG 10 8 17 8 24 0 100 5 10
    240605162645_4l98 AAACGTGCAGACCTTTGTAATGAGCGGTGCAG(+CAGTGT)GATCCATATAACCTGCACAAAAAGGCAAAGT NGG 10 8 17 8 24 0 100 5 10
    240605162644_w00t GAGTCCAGCATAACCAGAAAGTCCATGAGCTG(+CAGTGT)GTACCCCTGAAGCCTGGGTGTGCAGACGAGC NGG 10 8 17 8 24 0 100 5 10
    240605162642_wahu TAGAGGCTCGTCTGCACACCCAGGCTTCAGGG(+CAGTGT)GTACCAGCTCATGGACTTTCTGGTTATGCTG NGG 10 8 17 8 24 0 100 5 10
    240605162640_kgcl TGGCCCTTGCAGTACTGTAAGATTGTGATGGG(+CAGTGT)GATCCTTTTGTTTGTTTGACTAATGTCTCTC NGG 10 8 17 8 24 0 100 5 10
    240605162639_u8tg ATTCTGAGAGACATTAGTCAAACAAACAAAAG(+CAGTGT)GATCCCCATCACAATCTTACAGTACTGCAAG NGG 10 8 17 8 24 0 100 5 10