Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605162608_xscu CTTAGCATTTTTACCTGCTGTTTGGCTCTGTG(+CAGTGT)GTACCAAGTCATGGGAATTTCTCTCGGGGTC NGG 10 8 17 8 24 0 100 5 10
    240605162607_j1tx CATTTGACCCCGAGAGAAATTCCCATGACTTG(+CAGTGT)GTACCACAGAGCCAAACAGCAGGTAAAAATG NGG 10 8 17 8 24 0 100 5 10
    240605162604_1ubb AAGCATTCCCAGAGAGCAGTTGACTAGCGGGG(+CAGTGT)GATCCTTTAAAGGGCCAGGCACACAAAAGAA NGG 10 8 17 8 24 0 100 5 10
    240605162603_i3jt ATGATTTCTTTTGTGTGCCTGGCCCTTTAAAG(+CAGTGT)GATCCCCCGCTAGTCAACTGCTCTCTGGGAA NGG 10 8 17 8 24 0 100 5 10
    240605162600_1z7k GTATTTTCAGAGTCAGTTGACTAGCCATGGGG(+CAGTGT)GATCCTTTAAGGGGCCAGGCACGCACATCAT NGG 10 8 17 8 24 0 100 5 10
    240605162559_4010 GTAGAATGATGTGCGTGCCTGGCCCCTTAAAG(+CAGTGT)GATCCCCCATGGCTAGTCAACTGACTCTGAA NGG 10 8 17 8 24 0 100 5 10
    240605162557_a4pz CCTGATTCGTCCTGTTTATACTCCCCTCCAAG(+CAGTGT)GATCCTTCCCGATGCAGCAGGTGCGGGCGGC NGG 10 8 17 8 24 0 100 5 10
    240605162556_6swl GTGGGGCCGCCCGCACCTGCTGCATCGGGAAG(+CAGTGT)GATCCTTGGAGGGGAGTATAAACAGGACGAA NGG 10 8 17 8 24 0 100 5 10
    240605162553_yket CCTGGGTGGAAACACTGCCTTGTAGCTCTGGG(+CAGTGT)GTACCAGCCACAGATGATCCCTCTTGTACAA NGG 10 8 17 8 24 0 100 5 10
    240605162552_99i9 TTTATTTGTACAAGAGGGATCATCTGTGGCTG(+CAGTGT)GTACCCCAGAGCTACAAGGCAGTGTTTCCAC NGG 10 8 17 8 24 0 100 5 10