Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605162521_3u8w AGATCACAGTCTCTTACAACCATGCTTTTCAG(+CAGTGT)GATCCAGGAAGTAGTAAGTGTGCTGGGAGAT NGG 10 8 17 8 24 0 100 5 10
    240605162519_sk6r TGGGTATCTCCCAGCACACTTACTACTTCCTG(+CAGTGT)GATCCTGAAAAGCATGGTTGTAAGAGACTGT NGG 10 8 17 8 24 0 100 5 10
    240605162516_cdlb TGTAGCAGTTTTAACCAAATTTCATTCCCCTG(+CAGTGT)GTACCGTGGAGCTTTATTTTGCTATTGAACG NGG 10 8 17 8 24 0 100 5 10
    240605162514_rysi CTTGGCGTTCAATAGCAAAATAAAGCTCCACG(+CAGTGT)GTACCAGGGGAATGAAATTTGGTTAAAACTG NGG 10 8 17 8 24 0 100 5 10
    240605162512_9jvd GCTAGGAATACAGTGATGGAAAAATAAACAAG(+CAGTGT)GTACCTACTTCTTTGGAGACTGGAGTGGTTG NGG 10 8 17 8 24 0 100 5 10
    240605162511_ah8d GGTTCCAACCACTCCAGTCTCCAAAGAAGTAG(+CAGTGT)GTACCTTGTTTATTTTTCCATCACTGTATTC NGG 10 8 17 8 24 0 100 5 10
    240605162509_xaks TTCCTGACTTCCAGCCTTGCCTGACTCACCGG(+CAGTGT)GATCCTTATGTTTGGGCCTTCCCCATCCTAT NGG 10 8 17 8 24 0 100 5 10
    240605162508_k4q6 GTCCAATAGGATGGGGAAGGCCCAAACATAAG(+CAGTGT)GATCCCGGTGAGTCAGGCAAGGCTGGAAGTC NGG 10 8 17 8 24 0 100 5 10
    240605162506_leld CTGAGATTACAGAAGTACCCAGAAGTGTCTGG(+CAGTGT)GATCCCTGTCACATCTGTGTCATGTTGTTAT NGG 10 8 17 8 24 0 100 5 10
    240605162505_dcrk TTCAAATAACAACATGACACAGATGTGACAGG(+CAGTGT)GATCCCAGACACTTCTGGGTACTTCTGTAAT NGG 10 8 17 8 24 0 100 5 10