Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605162536_l4a0 TATAGGACTTGATGAAATTAAGCAAGGTCCTG(+CAGTGT)GATCCCAGAAACAGGTCCACAGGTTCTTCGG NGG 10 8 17 8 24 0 100 5 10
    240605162534_hknq GCATACCGAAGAACCTGTGGACCTGTTTCTGG(+CAGTGT)GATCCAGGACCTTGCTTAATTTCATCAAGTC NGG 10 8 17 8 24 0 100 5 10
    240605162533_4yxg CGATGGCTCTATTAATACACACACGGTGACAG(+CAGTGT)GTACCCTATTTAGACTAAGGCTACTAGAACA NGG 10 8 17 8 24 0 100 5 10
    240605162531_3ohz GAGTGTGTTCTAGTAGCCTTAGTCTAAATAGG(+CAGTGT)GTACCTGTCACCGTGTGTGTATTAATAGAGC NGG 10 8 17 8 24 0 100 5 10
    240605162528_0r7z GATACATCTACTCAGCATAGGAGAGCCCTGGG(+CAGTGT)GTACCACTTACAGGCTATCTAGAATAGTTCC NGG 10 8 17 8 24 0 100 5 10
    240605162527_ddau GCTGTGGAACTATTCTAGATAGCCTGTAAGTG(+CAGTGT)GTACCCCAGGGCTCTCCTATGCTGAGTAGAT NGG 10 8 17 8 24 0 100 5 10
    240605162525_c4j1 CATGTGTGCTCGTTGTCTTAAGTCCCTGGAGG(+CAGTGT)GTACCACACTGCTTTCATCCTGTCCCCTTGG NGG 10 8 17 8 24 0 100 5 10
    240605162524_53q1 TTTTTCCAAGGGGACAGGATGAAAGCAGTGTG(+CAGTGT)GTACCCTCCAGGGACTTAAGACAACGAGCAC NGG 10 8 17 8 24 0 100 5 10
    240605162523_i7bz ATAAAAAGAGTTCTGTGCTTGTGTGGAGATTG(+CAGTGT)GATCCGAAGAAACTGGCTGCTGGGGTTTGGC NGG 10 8 17 8 24 0 100 5 10
    240605162522_z69i CCCGGGCCAAACCCCAGCAGCCAGTTTCTTCG(+CAGTGT)GATCCAATCTCCACACAAGCACAGAACTCTT NGG 10 8 17 8 24 0 100 5 10