Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605162623_yhxr ATGACTGCTTTGACTGCTTATAAAAGTGACAG(+CAGTGT)GATCCTCAGTAAGCAGCAACTCGAGAACTTG NGG 10 8 17 8 24 0 100 5 10
    240605162622_l11m GTCCCCAAGTTCTCGAGTTGCTGCTTACTGAG(+CAGTGT)GATCCTGTCACTTTTATAAGCAGTCAAAGCA NGG 10 8 17 8 24 0 100 5 10
    240605162620_7055 GATAACTTCACAACATTGGAGTGGTCAGAAAG(+CAGTGT)GTACCTGTTAAATGTTACTAACGAAACAAAT NGG 10 8 17 8 24 0 100 5 10
    240605162619_agwn AGAGCATTTGTTTCGTTAGTAACATTTAACAG(+CAGTGT)GTACCTTTCTGACCACTCCAATGTTGTGAAG NGG 10 8 17 8 24 0 100 5 10
    240605162618_yzgt TTCCTCTATAAATTTTAAGTCTTGTTGCAGAG(+CAGTGT)GTACCCAGGAAGTCCTGCTGTCCTCTAATGG NGG 10 8 17 8 24 0 100 5 10
    240605162616_o03r ACAACCCATTAGAGGACAGCAGGACTTCCTGG(+CAGTGT)GTACCTCTGCAACAAGACTTAAAATTTATAG NGG 10 8 17 8 24 0 100 5 10
    240605162615_jpyu ACCGTGGTTTCTGCTTCAGCCCCTGCATCTAG(+CAGTGT)GATCCTGCTGTGTTTGAGATCTTGTCCTGAC NGG 10 8 17 8 24 0 100 5 10
    240605162613_4vxm AGGAAGTCAGGACAAGATCTCAAACACAGCAG(+CAGTGT)GATCCTAGATGCAGGGGCTGAAGCAGAAACC NGG 10 8 17 8 24 0 100 5 10
    240605162610_quh9 TCCTCCAACCACCTACAACTCCATCTCCAGGG(+CAGTGT)GATCCAATGCCTTCTTCTGGTCTTTGCAGGC NGG 10 8 17 8 24 0 100 5 10
    240605162609_6hvl CAGGTGCCTGCAAAGACCAGAAGAAGGCATTG(+CAGTGT)GATCCCCTGGAGATGGAGTTGTAGGTGGTTG NGG 10 8 17 8 24 0 100 5 10