Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605161327_9e0r AACTAGGCCCTAGGGAGGGCTACCCTGCGATG(+CAGTGT)GTACCCCAAGAGGCTCAGGTGTGTTCTGTAG NGG 10 8 17 8 24 0 100 5 10
    240605161326_30h3 CCAAGAACCAACTAGCTGCCTCAGGTCTCGTG(+CAGTGT)GATCCCAGATGGACATGGCAGTAACTCAGAG NGG 10 8 17 8 24 0 100 5 10
    240605161324_7100 ACCGGCTCTGAGTTACTGCCATGTCCATCTGG(+CAGTGT)GATCCACGAGACCTGAGGCAGCTAGTTGGTT NGG 10 8 17 8 24 0 100 5 10
    240605161322_if9s TCTCACCTGAGAAATGGTGCAAACCAACCAGG(+CAGTGT)GATCCTTAGGCTACTGAGAAGGCTGTCTGCT NGG 10 8 17 8 24 0 100 5 10
    240605161320_zk7y AGGTAAGCAGACAGCCTTCTCAGTAGCCTAAG(+CAGTGT)GATCCCTGGTTGGTTTGCACCATTTCTCAGG NGG 10 8 17 8 24 0 100 5 10
    240605161318_5xya GGGCCCTGTGACCTTCAACATCTGGCAGGGGG(+CAGTGT)GATCCTGGGACAGGGGGTGGAGAAGGCAGTT NGG 10 8 17 8 24 0 100 5 10
    240605161315_z5mh GTGTCAACTGCCTTCTCCACCCCCTGTCCCAG(+CAGTGT)GATCCCCCCTGCCAGATGTTGAAGGTCACAG NGG 10 8 17 8 24 0 100 5 10
    240605161314_d01l TCACTAAGAGGACTGAAGGATCTAGTTGAGAG(+CAGTGT)GATCCCTCATGAACGTCCACTACCAGCCAGA NGG 10 8 17 8 24 0 100 5 10
    240605161312_e4tf TGAGCTCTGGCTGGTAGTGGACGTTCATGAGG(+CAGTGT)GATCCTCTCAACTAGATCCTTCAGTCCTCTT NGG 10 8 17 8 24 0 100 5 10
    240605161310_x851 TGGATAGGGTTGGAGCTACTGGGACAGAGCTG(+CAGTGT)GATCCAGCCATGAGTCTTATGTCCTCCCAGA NGG 10 8 17 8 24 0 100 5 10