Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605161222_a1dm TGCTGCAGACTCTCCCCCAGCTTCTGTGGAGG(+CAGTGT)GTACCACTCCTGTGCTTGGGGATCAGGTCCA NGG 10 8 17 8 24 0 100 5 10
    240605161220_iyyh ACTATTAACTATAATAGAAGTCACTAGGAAGG(+CAGTGT)GTACCATGCTCTCAGAATCCCCACGTCCCAG NGG 10 8 17 8 24 0 100 5 10
    240605161219_a4bz CGTGGCTGGGACGTGGGGATTCTGAGAGCATG(+CAGTGT)GTACCCTTCCTAGTGACTTCTATTATAGTTA NGG 10 8 17 8 24 0 100 5 10
    240605161217_b1qa TGGTCCTTGGCACAGCCCCTCCCTCATGGCTG(+CAGTGT)GATCCTCAGATCATAATAGGGCCTTGGAGCA NGG 10 8 17 8 24 0 100 5 10
    240605161216_a3q4 AACCCTGCTCCAAGGCCCTATTATGATCTGAG(+CAGTGT)GATCCAGCCATGAGGGAGGGGCTGTGCCAAG NGG 10 8 17 8 24 0 100 5 10
    240605161214_021l TTCACCTTCCGGAGAAAACATTTCAAGCGGAG(+CAGTGT)GATCCGGTATCTCGGGATTCCGAAGTCTCCC NGG 10 8 17 8 24 0 100 5 10
    240605161213_cgeq ACACCGGGAGACTTCGGAATCCCGAGATACCG(+CAGTGT)GATCCTCCGCTTGAAATGTTTTCTCCGGAAG NGG 10 8 17 8 24 0 100 5 10
    240605161211_58nu ATTGCACTTATTTCTATGAACCCCATGGAGGG(+CAGTGT)GATCCCCAATGCTGCTGCCTCCAGGCGAGGC NGG 10 8 17 8 24 0 100 5 10
    240605161209_wn9q GTCATGCCTCGCCTGGAGGCAGCAGCATTGGG(+CAGTGT)GATCCCCTCCATGGGGTTCATAGAAATAAGT NGG 10 8 17 8 24 0 100 5 10
    240605161208_gtvk GGTACTTCCTGCAGAGCCTGACACCAGAGTTG(+CAGTGT)GATCCCTGGGACCCACATGATGAGAGATCCA NGG 10 8 17 8 24 0 100 5 10