Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605161135_9pnw ATATTAGGTGTCTTTGTTAGTGGTTGGAATTG(+CAGTGT)GATCCCTGAGAACCAGTGGGACACACACACA NGG 10 8 17 8 24 0 100 5 10
    240605161133_pkoo TGGCTTACAACTGTCTATACCTCAGTTCCAGG(+CAGTGT)GATCCAGTGCCACCTTATGGTTCCCACAGGC NGG 10 8 17 8 24 0 100 5 10
    240605161131_zrlx TGCATGCCTGTGGGAACCATAAGGTGGCACTG(+CAGTGT)GATCCCTGGAACTGAGGTATAGACAGTTGTA NGG 10 8 17 8 24 0 100 5 10
    240605161130_lzit CTATTTAACTTTCCCTTAAATTTTAAGATTTG(+CAGTGT)GATCCCATGTATGTCATCATGTCCCCTAGAA NGG 10 8 17 8 24 0 100 5 10
    240605161128_s9q8 AAATATTCTAGGGGACATGATGACATACATGG(+CAGTGT)GATCCAAATCTTAAAATTTAAGGGAAAGTTA NGG 10 8 17 8 24 0 100 5 10
    240605161127_tw2n GTAAAGAGCATGTAAATACCACAGGAAGACAG(+CAGTGT)GATCCCTGGAGACATTGAAGGAACAAGAGAG NGG 10 8 17 8 24 0 100 5 10
    240605161124_bk8l CCTCCCTCTCTTGTTCCTTCAATGTCTCCAGG(+CAGTGT)GATCCTGTCTTCCTGTGGTATTTACATGCTC NGG 10 8 17 8 24 0 100 5 10
    240605161123_dxwm TCGTAGTCGCTAAAATTCACTCAATTCACATG(+CAGTGT)GATCCCAGCTGTGTGTTGTAAGGCTGAGTCA NGG 10 8 17 8 24 0 100 5 10
    240605161122_ecj8 TCCACTGACTCAGCCTTACAACACACAGCTGG(+CAGTGT)GATCCATGTGAATTGAGTGAATTTTAGCGAC NGG 10 8 17 8 24 0 100 5 10
    240605161120_t6yk TCCACTTTGAACGTTTTCAGAAATCCCATAAG(+CAGTGT)GATCCTGGAGTGATAGATGATTTCTGTCTTG NGG 10 8 17 8 24 0 100 5 10