Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605161030_rpaw ACTATATTAAAAAAAACACAAAATAAAAAAGG(+CAGTGT)GATCCATCAACATATATTTTAGAAGTCCATC NGG 10 8 17 8 24 0 100 5 10
    240605161029_pk5a ACACCGCCACGCTGTTTTGTTTTGTTTCTTTG(+CAGTGT)GATCCTGGCCTGGCATGTCGATAGTCAGAAG NGG 10 8 17 8 24 0 100 5 10
    240605161027_e3am CTTATCTTCTGACTATCGACATGCCAGGCCAG(+CAGTGT)GATCCAAAGAAACAAAACAAAACAGCGTGGC NGG 10 8 17 8 24 0 100 5 10
    240605161025_6s54 TTACTTTCTGCTTCCTTCCTGGGAGTCCTAGG(+CAGTGT)GATCCTCTCTTCCCCAGCTCAGGGGACAACA NGG 10 8 17 8 24 0 100 5 10
    240605161024_v3cs GGATCTGTTGTCCCCTGAGCTGGGGAAGAGAG(+CAGTGT)GATCCCTAGGACTCCCAGGAAGGAAGCAGAA NGG 10 8 17 8 24 0 100 5 10
    240605161022_2qnp GTAGCCTCCTCTATAACAGGAGCAGGGAGAAG(+CAGTGT)GTACCAGTGCCTCACTACTAAATGTCCTAAT NGG 10 8 17 8 24 0 100 5 10
    240605161021_xxyi GGAAAATTAGGACATTTAGTAGTGAGGCACTG(+CAGTGT)GTACCTTCTCCCTGCTCCTGTTATAGAGGAG NGG 10 8 17 8 24 0 100 5 10
    240605161020_pm9b AATGTCCTAATTTTCCCCTCCATGGGAACTTG(+CAGTGT)GATCCAGGGTGGTGGCAAGTTACCCAAGGAC NGG 10 8 17 8 24 0 100 5 10
    240605161018_bv7v TTCATGTCCTTGGGTAACTTGCCACCACCCTG(+CAGTGT)GATCCAAGTTCCCATGGAGGGGAAAATTAGG NGG 10 8 17 8 24 0 100 5 10
    240605161015_9faf GTATTTATTAAATAGCTTCTATGGGCCCGGCG(+CAGTGT)GTACCTGTCTTTTTCGTCACTTTTCTTGCAG NGG 10 8 17 8 24 0 100 5 10