Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605161103_gkp2 GAGGGCAATTCCCATACGTAAAGACGGGAAAG(+CAGTGT)GTACCAGTCAAGGAGGAAAGCGGTCAATGCA NGG 10 8 17 8 24 0 100 5 10
    240605161101_pqho GAGCGCATCCCAGCATGGAGCAGGTAGAGTGG(+CAGTGT)GTACCAAGGCACACCCCTAGCTCAGAGGCTA NGG 10 8 17 8 24 0 100 5 10
    240605161059_17qe GCCAATAGCCTCTGAGCTAGGGGTGTGCCTTG(+CAGTGT)GTACCCACTCTACCTGCTCCATGCTGGGATG NGG 10 8 17 8 24 0 100 5 10
    240605161058_zh2l CGAGAGGTTTTCACAAACCGAGTCAAAACAAG(+CAGTGT)GTACCAGTTTTGAGCTCACATCAACTTTTTA NGG 10 8 17 8 24 0 100 5 10
    240605161057_cxi9 ACACTTAAAAAGTTGATGTGAGCTCAAAACTG(+CAGTGT)GTACCTTGTTTTGACTCGGTTTGTGAAAACC NGG 10 8 17 8 24 0 100 5 10
    240605161055_4mhk TCTCTGTCTTGAGAGACCAGAAGAGAGCATTG(+CAGTGT)GATCCCATTACAGATGATTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240605161054_zy1a ACCACATGGTGGCTCACAATCATCTGTAATGG(+CAGTGT)GATCCAATGCTCTCTTCTGGTCTCTCAAGAC NGG 10 8 17 8 24 0 100 5 10
    240605161051_5ez8 ATTGCAAGCAAGGGATTAAAGATTTGTCTTGG(+CAGTGT)GTACCAAATCACATAACGAGGATTCAAAGGA NGG 10 8 17 8 24 0 100 5 10
    240605161050_pyss GTTAATCCTTTGAATCCTCGTTATGTGATTTG(+CAGTGT)GTACCCAAGACAAATCTTTAATCCCTTGCTT NGG 10 8 17 8 24 0 100 5 10
    240605161049_zxl1 TGGATTCGTATGGCTAACGTCAGTGAATCAAG(+CAGTGT)GATCCCAGGGGATGCCCATTGCTGGGCATCT NGG 10 8 17 8 24 0 100 5 10