Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605161205_npgi GAAGCTGGATCTCTCATCATGTGGGTCCCAGG(+CAGTGT)GATCCAACTCTGGTGTCAGGCTCTGCAGGAA NGG 10 8 17 8 24 0 100 5 10
    240605161203_nz55 CAGATTGATGTTCAACATGGGGTCTGAGTAGG(+CAGTGT)GATCCCAACTAAAAAGCCATAACCTTCAGAA NGG 10 8 17 8 24 0 100 5 10
    240605161202_7e7z TTCAGTTCTGAAGGTTATGGCTTTTTAGTTGG(+CAGTGT)GATCCCTACTCAGACCCCATGTTGAACATCA NGG 10 8 17 8 24 0 100 5 10
    240605161200_yzbu TGTTGCTGTGAGGTCTTATGGCAGCTGCTCTG(+CAGTGT)GATCCATTGCTGGCTTCTTTGGCACCTCCAG NGG 10 8 17 8 24 0 100 5 10
    240605161159_1u0w TGAACCTGGAGGTGCCAAAGAAGCCAGCAATG(+CAGTGT)GATCCAGAGCAGCTGCCATAAGACCTCACAG NGG 10 8 17 8 24 0 100 5 10
    240605161158_dt2j GGTTTGTCCAAATGTGTGGCATGGGAGATAGG(+CAGTGT)GTACCCCTGAAAGCAAGGAAAGGAGCCCAGC NGG 10 8 17 8 24 0 100 5 10
    240605161156_uog3 CCACAGCTGGGCTCCTTTCCTTGCTTTCAGGG(+CAGTGT)GTACCCTATCTCCCATGCCACACATTTGGAC NGG 10 8 17 8 24 0 100 5 10
    240605161154_bb5m TACTGCCTTCCTGGGTTCCATGCCAGATGTTG(+CAGTGT)GATCCTAAGCAGGAGGTAGCTGAGTTGAGAT NGG 10 8 17 8 24 0 100 5 10
    240605161153_gt2g GGTCCATCTCAACTCAGCTACCTCCTGCTTAG(+CAGTGT)GATCCAACATCTGGCATGGAACCCAGGAAGG NGG 10 8 17 8 24 0 100 5 10
    240605161151_p9xs AATGCTTATGAATATTTGTGATCTATAGGTTG(+CAGTGT)GATCCATAGCAACACCAACACACAAAAAATG NGG 10 8 17 8 24 0 100 5 10