Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605161118_laru TTCACCAAGACAGAAATCATCTATCACTCCAG(+CAGTGT)GATCCTTATGGGATTTCTGAAAACGTTCAAA NGG 10 8 17 8 24 0 100 5 10
    240605161117_doi7 TGTGTATGTCTGTGTACCAGAAGACGACATTG(+CAGTGT)GATCCCATAGATCTGTTGTATGGATGGCTGT NGG 10 8 17 8 24 0 100 5 10
    240605161116_yb8b GACTTACAGCCATCCATACAACAGATCTATGG(+CAGTGT)GATCCAATGTCGTCTTCTGGTACACAGACAT NGG 10 8 17 8 24 0 100 5 10
    240605161113_xvil GTAAAGTAGTAGCTAACCCCTCCAAGGCCCAG(+CAGTGT)GATCCTAGTTCCAGTGTTACAGAAATGTTTC NGG 10 8 17 8 24 0 100 5 10
    240605161111_90vw AAAAGGAAACATTTCTGTAACACTGGAACTAG(+CAGTGT)GATCCTGGGCCTTGGAGGGGTTAGCTACTAC NGG 10 8 17 8 24 0 100 5 10
    240605161110_blfo CACTTTACTGTTTCAATGGTAATAATGCACCG(+CAGTGT)GTACCTCAATTCAACTGCTACAAATAAATGT NGG 10 8 17 8 24 0 100 5 10
    240605161108_uznc TTTTGACATTTATTTGTAGCAGTTGAATTGAG(+CAGTGT)GTACCGGTGCATTATTACCATTGAAACAGTA NGG 10 8 17 8 24 0 100 5 10
    240605161107_xcr9 ATGTATCCTTACTTTGACAATTAGACACACAG(+CAGTGT)GTACCAGGAAAAAAGGAATTGACTTTCAGGC NGG 10 8 17 8 24 0 100 5 10
    240605161105_k0ql TTCCAGCCTGAAAGTCAATTCCTTTTTTCCTG(+CAGTGT)GTACCTGTGTGTCTAATTGTCAAAGTAAGGA NGG 10 8 17 8 24 0 100 5 10
    240605161104_dh02 TGGTTTGCATTGACCGCTTTCCTCCTTGACTG(+CAGTGT)GTACCTTTCCCGTCTTTACGTATGGGAATTG NGG 10 8 17 8 24 0 100 5 10