Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605161014_lj6r TTAGGCTGCAAGAAAAGTGACGAAAAAGACAG(+CAGTGT)GTACCGCCGGGCCCATAGAAGCTATTTAATA NGG 10 8 17 8 24 0 100 5 10
    240605161012_4k82 AGGTGGTGTCTGCATTCCAACCACTGAATCCG(+CAGTGT)GATCCCATTTGTGAATAAGCCATTCAGACTC NGG 10 8 17 8 24 0 100 5 10
    240605161010_elv0 AATATGAGTCTGAATGGCTTATTCACAAATGG(+CAGTGT)GATCCGGATTCAGTGGTTGGAATGCAGACAC NGG 10 8 17 8 24 0 100 5 10
    240605161009_g006 GGAAACTGAAGTGTGTGCACACTTGAAGGAAG(+CAGTGT)GATCCCGAGTTTTCATATATTTTTAATATAT NGG 10 8 17 8 24 0 100 5 10
    240605161007_6pf3 TCTTAATATATTAAAAATATATGAAAACTCGG(+CAGTGT)GATCCTTCCTTCAAGTGTGCACACACTTCAG NGG 10 8 17 8 24 0 100 5 10
    240605161006_jxye ACAGACCAGGTAAAGAGAAAGCCCTAGTACAG(+CAGTGT)GTACCTTGCAATCAACCATGCTTGGTTCAAC NGG 10 8 17 8 24 0 100 5 10
    240605161005_lj70 TCAAGGTTGAACCAAGCATGGTTGATTGCAAG(+CAGTGT)GTACCTGTACTAGGGCTTTCTCTTTACCTGG NGG 10 8 17 8 24 0 100 5 10
    240605161003_nif8 CAGAGCCAACAGCTTCTCATTCTAAAGCAAGG(+CAGTGT)GATCCCTAGAGTTTGGAGCTTCATGTTGGGG NGG 10 8 17 8 24 0 100 5 10
    240605161001_0qzi CCTAACCCCAACATGAAGCTCCAAACTCTAGG(+CAGTGT)GATCCCTTGCTTTAGAATGAGAAGCTGTTGG NGG 10 8 17 8 24 0 100 5 10
    240605161000_9ssp GCCTCTGTGTAGTCTGGATCTACTGACACTGG(+CAGTGT)GTACCAGGGTTTCCTTCTGTTGACTGTGATT NGG 10 8 17 8 24 0 100 5 10