Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605161046_bdq4 AACTGAGATGCCCAGCAATGGGCATCCCCTGG(+CAGTGT)GATCCTTGATTCACTGACGTTAGCCATACGA NGG 10 8 17 8 24 0 100 5 10
    240605161045_qpei ATTGCAAGCAAGGGATTAAAGATTTGTCTTGG(+CAGTGT)GTACCAAATCACATAACGAGGATTCAAAGGA NGG 10 8 17 8 24 0 100 5 10
    240605161043_rx3d GTTAATCCTTTGAATCCTCGTTATGTGATTTG(+CAGTGT)GTACCCAAGACAAATCTTTAATCCCTTGCTT NGG 10 8 17 8 24 0 100 5 10
    240605161042_nduq TCTACATTTTAATTTTTATGATTCCTGATGTG(+CAGTGT)GATCCATTACAAACTTCTCAGAAGTTTGGAG NGG 10 8 17 8 24 0 100 5 10
    240605161041_qf1u ATGCACTCCAAACTTCTGAGAAGTTTGTAATG(+CAGTGT)GATCCACATCAGGAATCATAAAAATTAAAAT NGG 10 8 17 8 24 0 100 5 10
    240605161038_hkez GGAAGAAAGGTGGTGTCCTTCCGGACTCCGGG(+CAGTGT)GATCCAGGCCTGAATATGGGAGGCACCCAGG NGG 10 8 17 8 24 0 100 5 10
    240605161036_s4ln CTGAGCCTGGGTGCCTCCCATATTCAGGCCTG(+CAGTGT)GATCCCCGGAGTCCGGAAGGACACCACCTTT NGG 10 8 17 8 24 0 100 5 10
    240605161035_g1y5 AAAAATTACTAAAACCATGCAAAATGATTGAG(+CAGTGT)GATCCCAAAGGCTTTCTTAATGTACACTAGT NGG 10 8 17 8 24 0 100 5 10
    240605161033_2uk7 TAGATACTAGTGTACATTAAGAAAGCCTTTGG(+CAGTGT)GATCCTCAATCATTTTGCATGGTTTTAGTAA NGG 10 8 17 8 24 0 100 5 10
    240605161032_0vuj TCTTGGATGGACTTCTAAAATATATGTTGATG(+CAGTGT)GATCCCTTTTTTATTTTGTGTTTTTTTTAAT NGG 10 8 17 8 24 0 100 5 10