Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605160905_ln0x TGCAGGAAAGAGTAGACAGCATCAGAGGACTG(+CAGTGT)GTACCTGGGGTTTCCCTCTGGTTTACACACA NGG 10 8 17 8 24 0 100 5 10
    240605160903_vw8q ACCAGACATGTTCTAAGGCTGCAGTGCTGCAG(+CAGTGT)GTACCCTCACCTTGGAGGTCATGCTGGTGTT NGG 10 8 17 8 24 0 100 5 10
    240605160902_hf2h TCTAGAACACCAGCATGACCTCCAAGGTGAGG(+CAGTGT)GTACCTGCAGCACTGCAGCCTTAGAACATGT NGG 10 8 17 8 24 0 100 5 10
    240605160859_x98s TTCTTGGCATGAGCCTATCCTTATCGCCCTTG(+CAGTGT)GATCCCAGTTTAACAAGACCTTTTTCTAAAG NGG 10 8 17 8 24 0 100 5 10
    240605160857_n57u ATTGTCTTTAGAAAAAGGTCTTGTTAAACTGG(+CAGTGT)GATCCAAGGGCGATAAGGATAGGCTCATGCC NGG 10 8 17 8 24 0 100 5 10
    240605160856_t4nr TGCATCTTCATCTTCATCACGACACCAGACAG(+CAGTGT)GATCCAATCTTGGATGGAGACATTACCAGTG NGG 10 8 17 8 24 0 100 5 10
    240605160855_cnwf CCCTTCACTGGTAATGTCTCCATCCAAGATTG(+CAGTGT)GATCCTGTCTGGTGTCGTGATGAAGATGAAG NGG 10 8 17 8 24 0 100 5 10
    240605160853_t4t7 GTTGTTTGGTTTGTTTATTTCTGTGGTTCTGG(+CAGTGT)GATCCAGCCCAGGGCCTTACATGTGCTAGAC NGG 10 8 17 8 24 0 100 5 10
    240605160852_m5r9 TATTTGTCTAGCACATGTAAGGCCCTGGGCTG(+CAGTGT)GATCCCAGAACCACAGAAATAAACAAACCAA NGG 10 8 17 8 24 0 100 5 10
    240605160850_mk2n GTTACAGCAGGGATTTGACAAAACACAGGGTG(+CAGTGT)GATCCATGCAGTTTGGAAACTTAACTTTGAA NGG 10 8 17 8 24 0 100 5 10