Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605160802_euwi CCTCCCTGAACCCACTCCCGTTCTGGTAGCTG(+CAGTGT)GATCCCCTCCCTCTGATTTTGATATCATAGT NGG 10 8 17 8 24 0 100 5 10
    240605160800_4hqz TTGGACAGCCAGGGCTACACAGAGAGATCCTG(+CAGTGT)GATCCCGTCTTCCAAAAAAAAAAAAAAGAAA NGG 10 8 17 8 24 0 100 5 10
    240605160758_rr9t CTTTCTTTCTTTTTTTTTTTTTTGGAAGACGG(+CAGTGT)GATCCAGGATCTCTCTGTGTAGCCCTGGCTG NGG 10 8 17 8 24 0 100 5 10
    240605160757_yyi3 TCAGAAACTTCAGGCTCTGCTTGGAAAACTTG(+CAGTGT)GTACCACAAATGGGTGTCAAAGGTCATGTGT NGG 10 8 17 8 24 0 100 5 10
    240605160755_ucyq TTGTTACACATGACCTTTGACACCCATTTGTG(+CAGTGT)GTACCAAGTTTTCCAAGCAGAGCCTGAAGTT NGG 10 8 17 8 24 0 100 5 10
    240605160754_6m40 CTGGCCCCAGGCTGGGGTCCTATGCAAAGCAG(+CAGTGT)GATCCCACTAATGGGATTCAGGCAGAAGTGT NGG 10 8 17 8 24 0 100 5 10
    240605160752_hfnr GGTAGACACTTCTGCCTGAATCCCATTAGTGG(+CAGTGT)GATCCTGCTTTGCATAGGACCCCAGCCTGGG NGG 10 8 17 8 24 0 100 5 10
    240605160750_xrbo CGACTCAAAGGGCCTTCTTGCTGGGGCCTCTG(+CAGTGT)GATCCTCTGTCCTGGGGCCGGAAGGCAGGGG NGG 10 8 17 8 24 0 100 5 10
    240605160749_8lkv TCCGGCCCCTGCCTTCCGGCCCCAGGACAGAG(+CAGTGT)GATCCAGAGGCCCCAGCAAGAAGGCCCTTTG NGG 10 8 17 8 24 0 100 5 10
    240605160748_lcl2 TTGTCATTCATCACGAAGTAGGCCAAATCATG(+CAGTGT)GTACCACAGGAATTTAGAAATCTAACTGCAA NGG 10 8 17 8 24 0 100 5 10