Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605160711_j9ir GTATAAACACAGAAGGGGTGAAGAACCTGTGG(+CAGTGT)GTACCTTTCCAACCATATGAAGGACAGTCAT NGG 10 8 17 8 24 0 100 5 10
    240605160709_5nwz CCCTCCACCACTCCTGCGTGCGCATGCCTGTG(+CAGTGT)GTACCTTACAATCACCCCCCCCCCCTTCACA NGG 10 8 17 8 24 0 100 5 10
    240605160708_sa18 AATATTGTGAAGGGGGGGGGGGTGATTGTAAG(+CAGTGT)GTACCACAGGCATGCGCACGCAGGAGTGGTG NGG 10 8 17 8 24 0 100 5 10
    240605160706_zvb7 CAAACATTGTTTACCTTGTGTTTCACCCTTGG(+CAGTGT)GTACCACCCATCTACAAGTAGAAATCAGTAC NGG 10 8 17 8 24 0 100 5 10
    240605160704_qrfo GCCCAGTACTGATTTCTACTTGTAGATGGGTG(+CAGTGT)GTACCCAAGGGTGAAACACAAGGTAAACAAT NGG 10 8 17 8 24 0 100 5 10
    240605160702_iryg GGTGCTGCCCATGATACGGCACAGCCACAGTG(+CAGTGT)GTACCAGCATTGCTGCCATGTTGGCCATCCA NGG 10 8 17 8 24 0 100 5 10
    240605160701_twa3 CTGGCTGGATGGCCAACATGGCAGCAATGCTG(+CAGTGT)GTACCACTGTGGCTGTGCCGTATCATGGGCA NGG 10 8 17 8 24 0 100 5 10
    240605160659_d0cg AGCTTCCTCTCTGTAGTTCCCTGAGGTGGGAG(+CAGTGT)GTACCCACTTTGCCATGCATAACTCATCTCT NGG 10 8 17 8 24 0 100 5 10
    240605160657_jiyg GGAAGAGAGATGAGTTATGCATGGCAAAGTGG(+CAGTGT)GTACCTCCCACCTCAGGGAACTACAGAGAGG NGG 10 8 17 8 24 0 100 5 10
    240605160656_msiq CCTGTGCCCACTGGAGCCAGAAGAGGGCATTG(+CAGTGT)GATCCTGAAACTGAAGTTACAGATGTTGGGA NGG 10 8 17 8 24 0 100 5 10