Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605161701_jtej TCCCTGCAAGGCCAGCCCTGGGACAGTGCCTG(+CAGTGT)GTACCCGAGAAACAGTTATACGGGTGTCCTG NGG 10 8 17 8 24 0 100 5 10
    240605161659_h1gq CAGCGCAGGACACCCGTATAACTGTTTCTCGG(+CAGTGT)GTACCAGGCACTGTCCCAGGGCTGGCCTTGC NGG 10 8 17 8 24 0 100 5 10
    240605161656_ev3a AGATGGAGAGGACCTCAAGAGTCTGACCCTTG(+CAGTGT)GTACCATCCGATACTTAACTCCCTCGGTCCT NGG 10 8 17 8 24 0 100 5 10
    240605161655_c5xj GAAGAAGGACCGAGGGAGTTAAGTATCGGATG(+CAGTGT)GTACCAAGGGTCAGACTCTTGAGGTCCTCTC NGG 10 8 17 8 24 0 100 5 10
    240605161653_cc4j TTCTTCCTCTTCCTCTTCCTCCTTGAATCTGG(+CAGTGT)GTACCCTAACCCTGAGAAGAAAACCCTCAGT NGG 10 8 17 8 24 0 100 5 10
    240605161651_84lx AGAAAACTGAGGGTTTTCTTCTCAGGGTTAGG(+CAGTGT)GTACCCAGATTCAAGGAGGAAGAGGAAGAGG NGG 10 8 17 8 24 0 100 5 10
    240605161649_o6ll GCACAGCACCTACCAGCCCTGGCCCTTCCTTG(+CAGTGT)GTACCATCTCCACCACTGCTGGACTCCCACC NGG 10 8 17 8 24 0 100 5 10
    240605161647_x7j6 GGGTAGGTGGGAGTCCAGCAGTGGTGGAGATG(+CAGTGT)GTACCAAGGAAGGGCCAGGGCTGGTAGGTGC NGG 10 8 17 8 24 0 100 5 10
    240605161646_72ef ATATGATGAGCCCAAGAGATCTGGGATGAATG(+CAGTGT)GATCCCTAGATACGGTAGGCCCAGATCTCCC NGG 10 8 17 8 24 0 100 5 10
    240605161645_scri GGTGGGGGAGATCTGGGCCTACCGTATCTAGG(+CAGTGT)GATCCATTCATCCCAGATCTCTTGGGCTCAT NGG 10 8 17 8 24 0 100 5 10