Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605160727_mqn1 GGCGAGGACTGGTTTGACTCAGGGTTCCTTAG(+CAGTGT)GTACCACAGACAAAGGCCCCTGGCCTAGCTG NGG 10 8 17 8 24 0 100 5 10
    240605160726_05ot CGGGAGATATGAAGCCAAGCTGTGTAGTTCAG(+CAGTGT)GATCCCGAGACCCTAAACACCACACTGACCT NGG 10 8 17 8 24 0 100 5 10
    240605160724_kn0o AAAGAAGGTCAGTGTGGTGTTTAGGGTCTCGG(+CAGTGT)GATCCTGAACTACACAGCTTGGCTTCATATC NGG 10 8 17 8 24 0 100 5 10
    240605160722_5uqo TCCATCCAAGTGTGGAAGGACCTCCTCTGAGG(+CAGTGT)GATCCACTGTAGCGAATCCATCCCAACCCTC NGG 10 8 17 8 24 0 100 5 10
    240605160721_di2k TGAATGAGGGTTGGGATGGATTCGCTACAGTG(+CAGTGT)GATCCCTCAGAGGAGGTCCTTCCACACTTGG NGG 10 8 17 8 24 0 100 5 10
    240605160719_pp3k GGAGGTGTACTGGTGCTAATTCTACAGATAGG(+CAGTGT)GATCCCATGTGCAGTGCTCGTGGATGGGACC NGG 10 8 17 8 24 0 100 5 10
    240605160718_4rzx CGAGTGGTCCCATCCACGAGCACTGCACATGG(+CAGTGT)GATCCCTATCTGTAGAATTAGCACCAGTACA NGG 10 8 17 8 24 0 100 5 10
    240605160716_hiic GAGCTCCATTTTTACGCTAGGCATCCATCTCG(+CAGTGT)GTACCCACTAAGCCTTTATCTCTGACTCCAC NGG 10 8 17 8 24 0 100 5 10
    240605160714_m20w AGGGAGTGGAGTCAGAGATAAAGGCTTAGTGG(+CAGTGT)GTACCGAGATGGATGCCTAGCGTAAAAATGG NGG 10 8 17 8 24 0 100 5 10
    240605160713_v7dx TCGACATGACTGTCCTTCATATGGTTGGAAAG(+CAGTGT)GTACCCACAGGTTCTTCACCCCTTCTGTGTT NGG 10 8 17 8 24 0 100 5 10