Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605160746_558g CCAGATTGCAGTTAGATTTCTAAATTCCTGTG(+CAGTGT)GTACCATGATTTGGCCTACTTCGTGATGAAT NGG 10 8 17 8 24 0 100 5 10
    240605160744_srnj AAAGCCCTGGGTTGACTGACTCCCTCACTCAG(+CAGTGT)GATCCTAAGAACAATGCTGACTGCAGTTTTC NGG 10 8 17 8 24 0 100 5 10
    240605160742_l2qb AAGTAGAAAACTGCAGTCAGCATTGTTCTTAG(+CAGTGT)GATCCTGAGTGAGGGAGTCAGTCAACCCAGG NGG 10 8 17 8 24 0 100 5 10
    240605160740_3nem GTGCAAGCGCCATGGCACCCTGCTGTACCACG(+CAGTGT)GTACCAAACTCAACTGTACAGTCAACAGTCA NGG 10 8 17 8 24 0 100 5 10
    240605160739_sd5i CTCGCTGACTGTTGACTGTACAGTTGAGTTTG(+CAGTGT)GTACCGTGGTACAGCAGGGTGCCATGGCGCT NGG 10 8 17 8 24 0 100 5 10
    240605160737_vtm9 CGTAGGCCGATGAGTGTAAGTAAAAGAGTGTG(+CAGTGT)GTACCACTGGAAAAAACTCCAAACCAGGGCC NGG 10 8 17 8 24 0 100 5 10
    240605160735_ttci TCGATGGCCCTGGTTTGGAGTTTTTTCCAGTG(+CAGTGT)GTACCACACTCTTTTACTTACACTCATCGGC NGG 10 8 17 8 24 0 100 5 10
    240605160732_kos1 AGAAGGCCCCTCTGAGTATTTATTTGACCGGG(+CAGTGT)GTACCAATGGTACAGCTATATATATGTACAG NGG 10 8 17 8 24 0 100 5 10
    240605160730_wzg6 TTACTCTGTACATATATATAGCTGTACCATTG(+CAGTGT)GTACCCCGGTCAAATAAATACTCAGAGGGGC NGG 10 8 17 8 24 0 100 5 10
    240605160729_zzfs AAGGACAGCTAGGCCAGGGGCCTTTGTCTGTG(+CAGTGT)GTACCTAAGGAACCCTGAGTCAAACCAGTCC NGG 10 8 17 8 24 0 100 5 10