Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605160926_fn2r GCCTACCTGGTATATCCCAGCAGCCACTTATG(+CAGTGT)GTACCCGTTCACTGCCTCTGGCATGCTTCAC NGG 10 8 17 8 24 0 100 5 10
    240605160922_m9lx GAGGGTTTTCAGTTGTGACAGCCCCACCCCCG(+CAGTGT)GTACCATCTCTCTCCTCAATGTAGTCATGCA NGG 10 8 17 8 24 0 100 5 10
    240605160921_8u44 GTGCTTGCATGACTACATTGAGGAGAGAGATG(+CAGTGT)GTACCGGGGGTGGGGCTGTCACAACTGAAAA NGG 10 8 17 8 24 0 100 5 10
    240605160919_pzkl CTGGGGCCAATAAGCTGGCTTAGTAGGTAAAG(+CAGTGT)GTACCTGCTGCCAAGCCTGATGCCCTGAGTT NGG 10 8 17 8 24 0 100 5 10
    240605160917_hsri GATTGAACTCAGGGCATCAGGCTTGGCAGCAG(+CAGTGT)GTACCTTTACCTACTAAGCCAGCTTATTGGC NGG 10 8 17 8 24 0 100 5 10
    240605160916_3kzq TTGCCTGCGTTTATGTCTGTGTGAGAGTTTTG(+CAGTGT)GATCCCCTAAAACGAGTTGCAAGCAGGTGTG NGG 10 8 17 8 24 0 100 5 10
    240605160913_3w3w TAACTCACACCTGCTTGCAACTCGTTTTAGGG(+CAGTGT)GATCCAAAACTCTCACACAGACATAAACGCA NGG 10 8 17 8 24 0 100 5 10
    240605160910_fvou AATAAGCCAGGCATGGTGCCTGGGGGCAGGAG(+CAGTGT)GATCCTGACTAGAAAGCGATTGGTCTGCATA NGG 10 8 17 8 24 0 100 5 10
    240605160909_grhy CTCACTATGCAGACCAATCGCTTTCTAGTCAG(+CAGTGT)GATCCTCCTGCCCCCAGGCACCATGCCTGGC NGG 10 8 17 8 24 0 100 5 10
    240605160906_rxq6 GTGTGTGTGTGTAAACCAGAGGGAAACCCCAG(+CAGTGT)GTACCAGTCCTCTGATGCTGTCTACTCTTTC NGG 10 8 17 8 24 0 100 5 10