Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605160516_ildm GTGGGTGTGAACTCTCACCTAGAAAACCTAGG(+CAGTGT)GTACCTTTGAAACCTGTAATGGTCATTTATT NGG 10 8 17 8 24 0 100 5 10
    240605160514_t9zl GGGCTGAGTGGAGACCCAGTTGGAGGAGGCTG(+CAGTGT)GATCCTTTGTTAGGAAGGCAAAGGCCCGGGG NGG 10 8 17 8 24 0 100 5 10
    240605160513_6ctp AGGTTCCCCGGGCCTTTGCCTTCCTAACAAAG(+CAGTGT)GATCCAGCCTCCTCCAACTGGGTCTCCACTC NGG 10 8 17 8 24 0 100 5 10
    240605160512_1pv6 TTATCAGCTTTCATTCAGTCTTCCTTTCTCTG(+CAGTGT)GATCCAATTATCTCACCTCCTTTAGCTATTC NGG 10 8 17 8 24 0 100 5 10
    240605160511_c4ay TTATGGAATAGCTAAAGGAGGTGAGATAATTG(+CAGTGT)GATCCAGAGAAAGGAAGACTGAATGAAAGCT NGG 10 8 17 8 24 0 100 5 10
    240605160509_j0mf CTACAATTCTATTTTAATCAGCTTTTTGGCTG(+CAGTGT)GTACCTACTGCTATACCACCTAGTATGACAT NGG 10 8 17 8 24 0 100 5 10
    240605160507_2s5s CCGAAATGTCATACTAGGTGGTATAGCAGTAG(+CAGTGT)GTACCAGCCAAAAAGCTGATTAAAATAGAAT NGG 10 8 17 8 24 0 100 5 10
    240605160505_c0iv TTTTTTATTCCTTCTCTGGCAATTCTTTCCTG(+CAGTGT)GATCCTCTGCTTAGCGAAATTATACCTACAG NGG 10 8 17 8 24 0 100 5 10
    240605160504_wrqn TTCCACTGTAGGTATAATTTCGCTAAGCAGAG(+CAGTGT)GATCCAGGAAAGAATTGCCAGAGAAGGAATA NGG 10 8 17 8 24 0 100 5 10
    240605160503_0iel AACACGGTCAGAGGCTCCCCAGAGCTGTGGTG(+CAGTGT)GTACCACCCATGTCTGAGATACTCTGTTGGA NGG 10 8 17 8 24 0 100 5 10