Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605160445_km5o TAGATGACCTATGCAATAAACAAAATCCTATG(+CAGTGT)GTACCCAAGTTGGTCAGTCAACCTCCTACTT NGG 10 8 17 8 24 0 100 5 10
    240605160443_haez GCTATGCACCCCTCTCCCTGGCACTGACCCTG(+CAGTGT)GATCCCCATCTTCTGTACCCAGTGACTAGAC NGG 10 8 17 8 24 0 100 5 10
    240605160441_mz1a CAGTCGTCTAGTCACTGGGTACAGAAGATGGG(+CAGTGT)GATCCAGGGTCAGTGCCAGGGAGAGGGGTGC NGG 10 8 17 8 24 0 100 5 10
    240605160438_61sz TTCCTGCCAAACTACCTTGCGAGGTTTCCTGG(+CAGTGT)GTACCCAAGTGGAGCCAAAGCTCACTAGGAC NGG 10 8 17 8 24 0 100 5 10
    240605160437_m39v GTCCTGTCCTAGTGAGCTTTGGCTCCACTTGG(+CAGTGT)GTACCCAGGAAACCTCGCAAGGTAGTTTGGC NGG 10 8 17 8 24 0 100 5 10
    240605160435_j6en CATGGAGAGACTTAATAAAGGGTTTTACAAGG(+CAGTGT)GTACCTGGATCCCAGGGGGAAGTAGTTTATC NGG 10 8 17 8 24 0 100 5 10
    240605160433_uw44 CCAAGGATAAACTACTTCCCCCTGGGATCCAG(+CAGTGT)GTACCCTTGTAAAACCCTTTATTAAGTCTCT NGG 10 8 17 8 24 0 100 5 10
    240605160431_3gyk AGACTTAATAAAGGGTTTTACAAGGGTACCTG(+CAGTGT)GATCCCAGGGGGAAGTAGTTTATCCTTGGGG NGG 10 8 17 8 24 0 100 5 10
    240605160428_swmu CTGTGCCCCAAGGATAAACTACTTCCCCCTGG(+CAGTGT)GATCCAGGTACCCTTGTAAAACCCTTTATTA NGG 10 8 17 8 24 0 100 5 10
    240605160426_vgoy TCTCAAAGATGAGGCCCACATGAACCTGCTGG(+CAGTGT)GATCCCACGAACACATATTGGACCTGAGCAC NGG 10 8 17 8 24 0 100 5 10