Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605155758_cmdm CAGGCAGAGAATGGTGCTCCACTGGGCTTCAG(+CAGTGT)GATCCAAACTTGGTCCTTTCTAGGTAAATGG NGG 10 8 17 8 24 0 100 5 10
    240605155756_ds3s TGAGAAGAGCCTCTGACAGGCATCAGAGCTGG(+CAGTGT)GATCCTGACCAAGGGACACTCAGGCTGCCAC NGG 10 8 17 8 24 0 100 5 10
    240605155755_8bzf AGCTGGTGGCAGCCTGAGTGTCCCTTGGTCAG(+CAGTGT)GATCCCAGCTCTGATGCCTGTCAGAGGCTCT NGG 10 8 17 8 24 0 100 5 10
    240605155752_rdl0 ACAGATAGTGTATGAAATACACAATTTCCCAG(+CAGTGT)GTACCAAGGGCCAATAGCAAAAAGGGTCTCT NGG 10 8 17 8 24 0 100 5 10
    240605155750_4jqm CTTTTAGAGACCCTTTTTGCTATTGGCCCTTG(+CAGTGT)GTACCTGGGAAATTGTGTATTTCATACACTA NGG 10 8 17 8 24 0 100 5 10
    240605155747_omz5 TTGACTGTCTCTGCATAGTCTTGATACCCCAG(+CAGTGT)GATCCTGACATCCCCAGAAATTATACCCCGC NGG 10 8 17 8 24 0 100 5 10
    240605155746_1ctc GGGGGGCGGGGTATAATTTCTGGGGATGTCAG(+CAGTGT)GATCCTGGGGTATCAAGACTATGCAGAGACA NGG 10 8 17 8 24 0 100 5 10
    240605155744_sjup TGCACACAGTGAGGAATAAACATTCTGGAAAG(+CAGTGT)GATCCTTGATTCTCCCACAAACTGTTCCTTG NGG 10 8 17 8 24 0 100 5 10
    240605155743_zns2 TACTCCAAGGAACAGTTTGTGGGAGAATCAAG(+CAGTGT)GATCCTTTCCAGAATGTTTATTCCTCACTGT NGG 10 8 17 8 24 0 100 5 10
    240605155742_gme7 TTTCCTTTTTAAATCATCCTGTGTGCATTCAG(+CAGTGT)GATCCTGGTCTTCATACAGAGATGTTGACAC NGG 10 8 17 8 24 0 100 5 10