Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605155814_5hvt GCTACTTCAATAGGACAGGCAGCCGGGCATTG(+CAGTGT)GTACCAATGCCATGACTGCCCAGACTCTTGT NGG 10 8 17 8 24 0 100 5 10
    240605155812_8yeb AGGACCCCCTCTTCCCCCAGAGGGGTCTCCTG(+CAGTGT)GATCCCCTTTCCTGTGAATGGAGGCTCCAAT NGG 10 8 17 8 24 0 100 5 10
    240605155810_ok0n ACTTTATTGGAGCCTCCATTCACAGGAAAGGG(+CAGTGT)GATCCAGGAGACCCCTCTGGGGGAAGAGGGG NGG 10 8 17 8 24 0 100 5 10
    240605155809_6xq3 AGAAGGCCATTCTGGAAAAAACAATAAAGTAG(+CAGTGT)GTACCACAACCCTGTGCCCACAGAACTTCCT NGG 10 8 17 8 24 0 100 5 10
    240605155807_25mp TGGCAAGGAAGTTCTGTGGGCACAGGGTTGTG(+CAGTGT)GTACCTACTTTATTGTTTTTTCCAGAATGGC NGG 10 8 17 8 24 0 100 5 10
    240605155806_yjnj CTCTTCTCTCCTCTTTCTTTGATACTTCATGG(+CAGTGT)GTACCCAAAGGATGCTCTGAAGTCACAGAAG NGG 10 8 17 8 24 0 100 5 10
    240605155804_a28h CTTTGCTTCTGTGACTTCAGAGCATCCTTTGG(+CAGTGT)GTACCCATGAAGTATCAAAGAAAGAGGAGAG NGG 10 8 17 8 24 0 100 5 10
    240605155802_livu GGCAGTTTCCTGAGACTGTAAAGTATTCCAGG(+CAGTGT)GATCCTTTTCCTGTGCGGATGCAGAAGCCAT NGG 10 8 17 8 24 0 100 5 10
    240605155800_9n8m CTGGCATGGCTTCTGCATCCGCACAGGAAAAG(+CAGTGT)GATCCCTGGAATACTTTACAGTCTCAGGAAA NGG 10 8 17 8 24 0 100 5 10
    240605155759_kgt6 CAACACCATTTACCTAGAAAGGACCAAGTTTG(+CAGTGT)GATCCTGAAGCCCAGTGGAGCACCATTCTCT NGG 10 8 17 8 24 0 100 5 10