Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605155916_nfc2 GTATCAAAATGCATCCTTTGTGATTCTCCGTG(+CAGTGT)GATCCTGCCTCCCTCATGATTACTCTAGCTT NGG 10 8 17 8 24 0 100 5 10
    240605155914_u57j GTATGCTGAGGTGTGGGGGTGGTGAATGAAGG(+CAGTGT)GTACCCTACCTGCAGTGCTCAAGAATGTCCA NGG 10 8 17 8 24 0 100 5 10
    240605155913_zr7x ATCATTGGACATTCTTGAGCACTGCAGGTAGG(+CAGTGT)GTACCCTTCATTCACCACCCCCACACCTCAG NGG 10 8 17 8 24 0 100 5 10
    240605155911_jchh TTAATCCCAGCACTAAGGAGGCAGAGCAGGCG(+CAGTGT)GATCCCAGTGAGTTTGAAGCCTGAGCTATAG NGG 10 8 17 8 24 0 100 5 10
    240605155910_lsto TCATCCTATAGCTCAGGCTTCAAACTCACTGG(+CAGTGT)GATCCGCCTGCTCTGCCTCCTTAGTGCTGGG NGG 10 8 17 8 24 0 100 5 10
    240605155908_mlca GGACAGAAGCAGTTTGAGCAGGTGTCTATATG(+CAGTGT)GTACCCTGCATCCGGGGCCCTTTGGGAACTG NGG 10 8 17 8 24 0 100 5 10
    240605155907_aqel CCGGCCAGTTCCCAAAGGGCCCCGGATGCAGG(+CAGTGT)GTACCATATAGACACCTGCTCAAACTGCTTC NGG 10 8 17 8 24 0 100 5 10
    240605155905_10wy CACATTTCTAAAAATCTTTCAATGACAAGTTG(+CAGTGT)GTACCCTTTAGCTAACTAAAGCTAAAAGGTG NGG 10 8 17 8 24 0 100 5 10
    240605155904_513f CCTTCCACCTTTTAGCTTTAGTTAGCTAAAGG(+CAGTGT)GTACCAACTTGTCATTGAAAGATTTTTAGAA NGG 10 8 17 8 24 0 100 5 10
    240605155902_m7h3 TCCTCCTATTCCTCCTGTGAGTTAGTGGCAGG(+CAGTGT)GTACCAACCCACTGGAGGGCAACAGGGAGGG NGG 10 8 17 8 24 0 100 5 10