Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605160021_9tzz AGTGTGTATTCCCCGTCCCCTCTGACACCTGG(+CAGTGT)GATCCAGATTTTGAATTGTGGCCCTGCTGGA NGG 10 8 17 8 24 0 100 5 10
    240605160020_4lj6 TTAAAAGAGTAGCTTCCTCTGCTGTACTAGTG(+CAGTGT)GATCCTGTTTTTGTTGCTTCCAGGGACCAGC NGG 10 8 17 8 24 0 100 5 10
    240605160019_rpcs AAAAAGCTGGTCCCTGGAAGCAACAAAAACAG(+CAGTGT)GATCCACTAGTACAGCAGAGGAAGCTACTCT NGG 10 8 17 8 24 0 100 5 10
    240605160016_ntdg TTTACATGAAGTGATGAGACCAAAGGCCCAAG(+CAGTGT)GATCCCCGTCCCCACCCCCCACCCCCTTTTG NGG 10 8 17 8 24 0 100 5 10
    240605160014_t174 GAAAACAAAAGGGGGTGGGGGGTGGGGACGGG(+CAGTGT)GATCCTTGGGCCTTTGGTCTCATCACTTCAT NGG 10 8 17 8 24 0 100 5 10
    240605160013_czq4 CAGAGCCAGTTTGGTCTTTCTCCCCCTGCCAG(+CAGTGT)GATCCCAGGCCCTAATCAGTATTATTTCTTT NGG 10 8 17 8 24 0 100 5 10
    240605160010_y1im TTATGAAAGAAATAATACTGATTAGGGCCTGG(+CAGTGT)GATCCTGGCAGGGGGAGAAAGACCAAACTGG NGG 10 8 17 8 24 0 100 5 10
    240605160009_ky6o AGCTTCAGGGTCCTCCTCGCTGTCCACACATG(+CAGTGT)GATCCCAGCGGAATCTCCGCTGACACGGAAG NGG 10 8 17 8 24 0 100 5 10
    240605160007_q74f TTTGCCTTCCGTGTCAGCGGAGATTCCGCTGG(+CAGTGT)GATCCATGTGTGGACAGCGAGGAGGACCCTG NGG 10 8 17 8 24 0 100 5 10
    240605160005_fapx TAGGCCCCTCCCAGCTGTGAGGGTCCAGGGAG(+CAGTGT)GATCCTGTGGCCGAGTCTTCAGTGGCCGAGT NGG 10 8 17 8 24 0 100 5 10