Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605160053_gkeo TGGATACAGATAGCACAATCCACTACCCAGTG(+CAGTGT)GATCCCTCAGCTGAAGGTTACTGATACCTAA NGG 10 8 17 8 24 0 100 5 10
    240605160051_xjcw GCCCCTGATCCAAGTCTCCAAAAGAGGCCAGG(+CAGTGT)GATCCTTCGAGCAAAGAGATAAATCTTATTC NGG 10 8 17 8 24 0 100 5 10
    240605160049_6tqc CACTTGAATAAGATTTATCTCTTTGCTCGAAG(+CAGTGT)GATCCCTGGCCTCTTTTGGAGACTTGGATCA NGG 10 8 17 8 24 0 100 5 10
    240605160048_cop7 TGAGCCCAGCATGGGCGATGGTCATCCTCATG(+CAGTGT)GATCCAGGATAGATGGTCACTCTCAGCATGG NGG 10 8 17 8 24 0 100 5 10
    240605160047_sq22 CCCAGCCATGCTGAGAGTGACCATCTATCCTG(+CAGTGT)GATCCATGAGGATGACCATCGCCCATGCTGG NGG 10 8 17 8 24 0 100 5 10
    240605160045_x3u0 GGAGATGTTTTTATAAAGCTGATGGCTCCTAG(+CAGTGT)GATCCCTGTGTTTGTCTACAGCTCAACACAG NGG 10 8 17 8 24 0 100 5 10
    240605160043_aguz TACTACTGTGTTGAGCTGTAGACAAACACAGG(+CAGTGT)GATCCTAGGAGCCATCAGCTTTATAAAAACA NGG 10 8 17 8 24 0 100 5 10
    240605160041_228v GATAGGGAGGGCATGCCTCGCTTTGGATGAGG(+CAGTGT)GATCCATTTAAACACTACCGTTTGGAATTGA NGG 10 8 17 8 24 0 100 5 10
    240605160040_bd7m AGCCCTCAATTCCAAACGGTAGTGTTTAAATG(+CAGTGT)GATCCCTCATCCAAAGCGAGGCATGCCCTCC NGG 10 8 17 8 24 0 100 5 10
    240605160038_xr6l ATCCTCTGCCCGCAATGCTTTTGAAAGACTTG(+CAGTGT)GATCCTTTTGAAATGGATGCTGAGCTGCACT NGG 10 8 17 8 24 0 100 5 10