Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605160141_n63m ATCCTGGTTCCTCTTGCCTGCTTAAAAACTGG(+CAGTGT)GATCCCGACTCCATGGATCAAAACTGAGTGA NGG 10 8 17 8 24 0 100 5 10
    240605160139_mtac CCAGCACTTTTGTTCACTTAAAATGTTGGAAG(+CAGTGT)GATCCAGGCCCTTTTCAGGAAGAAGCTGCCC NGG 10 8 17 8 24 0 100 5 10
    240605160137_i1ha TCTGGGGGCAGCTTCTTCCTGAAAAGGGCCTG(+CAGTGT)GATCCTTCCAACATTTTAAGTGAACAAAAGT NGG 10 8 17 8 24 0 100 5 10
    240605160135_k5us AAAAAAAAAACAGAAACAAAAAATGGTGCCAG(+CAGTGT)GTACCACAAGAGTAACAGAACTGTGCTGTTT NGG 10 8 17 8 24 0 100 5 10
    240605160134_u8ul CCAGAAAACAGCACAGTTCTGTTACTCTTGTG(+CAGTGT)GTACCTGGCACCATTTTTTGTTTCTGTTTTT NGG 10 8 17 8 24 0 100 5 10
    240605160133_efe5 AGGCCACTTCTCCAAGACAGTTTCTGACACTG(+CAGTGT)GTACCTTCCCCTAATCATGTATCTCCCAAAA NGG 10 8 17 8 24 0 100 5 10
    240605160131_6i2k TCATCTTTTGGGAGATACATGATTAGGGGAAG(+CAGTGT)GTACCAGTGTCAGAAACTGTCTTGGAGAAGT NGG 10 8 17 8 24 0 100 5 10
    240605160130_rw8x GCTTATCCAGGGCTCACTTTCGTCCTTTGTAG(+CAGTGT)GTACCCGCATTCCTACGCATTCATATACATA NGG 10 8 17 8 24 0 100 5 10
    240605160128_5qug GTTTGTATGTATATGAATGCGTAGGAATGCGG(+CAGTGT)GTACCTACAAAGGACGAAAGTGAGCCCTGGA NGG 10 8 17 8 24 0 100 5 10
    240605160127_wor5 CAGATTTTAGGTAAGTTTATTTGACTAAATTG(+CAGTGT)GATCCTTTTATATTAACAATTCAGCATCTTT NGG 10 8 17 8 24 0 100 5 10