Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605160246_sye4 TTACGGTGCTAGAGTGGGATCTTCTGTGTTAG(+CAGTGT)GATCCTTCTGGGGAAGCAGAATGAGTGTAGG NGG 10 8 17 8 24 0 100 5 10
    240605160244_4wh5 TATTGAATTTCTTCAGCAGCCAATGAATCCTG(+CAGTGT)GATCCATACAAAAATATATTTAGCTTCCACC NGG 10 8 17 8 24 0 100 5 10
    240605160243_kddz TTGCAGGTGGAAGCTAAATATATTTTTGTATG(+CAGTGT)GATCCAGGATTCATTGGCTGCTGAAGAAATT NGG 10 8 17 8 24 0 100 5 10
    240605160241_yv6k GAAGATGTTAAGGGAGGGTAGTTAAATCATGG(+CAGTGT)GTACCTAAGCTGGGGTTTTAGATTGAAGATG NGG 10 8 17 8 24 0 100 5 10
    240605160240_ubx6 TTGGGCATCTTCAATCTAAAACCCCAGCTTAG(+CAGTGT)GTACCCATGATTTAACTACCCTCCCTTAACA NGG 10 8 17 8 24 0 100 5 10
    240605160238_skju TTGAGGTATTCGTTGGGGGAGTGATGGAGGAG(+CAGTGT)GATCCAAAATGATTCATTGTATGGATGGCAG NGG 10 8 17 8 24 0 100 5 10
    240605160237_mvk1 CCTTTCTGCCATCCATACAATGAATCATTTTG(+CAGTGT)GATCCTCCTCCATCACTCCCCCAACGAATAC NGG 10 8 17 8 24 0 100 5 10
    240605160235_wako TTATACCACTAAGAAACCATGTACATAAATGG(+CAGTGT)GATCCAAATATAGTTTGGAGACAACCCAACT NGG 10 8 17 8 24 0 100 5 10
    240605160234_41df ATATTAGTTGGGTTGTCTCCAAACTATATTTG(+CAGTGT)GATCCCATTTATGTACATGGTTTCTTAGTGG NGG 10 8 17 8 24 0 100 5 10
    240605160232_z68d AGAAACAGATTTGTATTGTGTTTTATGGCCTG(+CAGTGT)GATCCAGGAGTACTTTTATAATGGGTAAGAT NGG 10 8 17 8 24 0 100 5 10