Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605160158_kiyn GCAGCAATGACCCTACTCCTCCTGTCAGCTAG(+CAGTGT)GATCCATGGGTTTCCCTAAATTTCCCAATGG NGG 10 8 17 8 24 0 100 5 10
    240605160157_064w ATTGTGCCTTCTGGACCAGGAGAGTAGCTTCG(+CAGTGT)GATCCCCTTGGCTGGTCCAACCTCTACTCCA NGG 10 8 17 8 24 0 100 5 10
    240605160155_oo1v CAGCCTGGAGTAGAGGTTGGACCAGCCAAGGG(+CAGTGT)GATCCGAAGCTACTCTCCTGGTCCAGAAGGC NGG 10 8 17 8 24 0 100 5 10
    240605160153_hmsf AAGGGCGGGGCTGGTTTGGCTTTTGTTGTTGG(+CAGTGT)GATCCCCGTGAGTGACAGGTTTTGCCGTGCA NGG 10 8 17 8 24 0 100 5 10
    240605160151_ewty TCCCGTGCACGGCAAAACCTGTCACTCACGGG(+CAGTGT)GATCCCAACAACAAAAGCCAAACCAGCCCCG NGG 10 8 17 8 24 0 100 5 10
    240605160150_015z CCATTCTTCCTCTTCATTGGATAGTTAGGCAG(+CAGTGT)GATCCTAGGAGCTGCTGCCAGTAGAGAACTG NGG 10 8 17 8 24 0 100 5 10
    240605160148_4s38 ACAGACAGTTCTCTACTGGCAGCAGCTCCTAG(+CAGTGT)GATCCTGCCTAACTATCCAATGAAGAGGAAG NGG 10 8 17 8 24 0 100 5 10
    240605160146_qon1 GAACTAGGTTAGATACAAATGGGCAAATACTG(+CAGTGT)GTACCAGGGTCTGGAGAAGGGCAGGAAAGTG NGG 10 8 17 8 24 0 100 5 10
    240605160144_0zc2 TAAAACACTTTCCTGCCCTTCTCCAGACCCTG(+CAGTGT)GTACCAGTATTTGCCCATTTGTATCTAACCT NGG 10 8 17 8 24 0 100 5 10
    240605160142_wqv0 CCTTGTCACTCAGTTTTGATCCATGGAGTCGG(+CAGTGT)GATCCCAGTTTTTAAGCAGGCAAGAGGAACC NGG 10 8 17 8 24 0 100 5 10