Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605160215_88ac GTTCCCCATGAGCATTATTCTCACAGAATAAG(+CAGTGT)GTACCACCACTCAAATGAATAAGAGAAGGAA NGG 10 8 17 8 24 0 100 5 10
    240605160213_8yn7 GTGTTGTTGTTGTAGTTTGTTTCTATGCCGTG(+CAGTGT)GTACCGTGGTTATCTCCCATGGAGGACTCCG NGG 10 8 17 8 24 0 100 5 10
    240605160211_vcrt CAGCCCGGAGTCCTCCATGGGAGATAACCACG(+CAGTGT)GTACCACGGCATAGAAACAAACTACAACAAC NGG 10 8 17 8 24 0 100 5 10
    240605160210_os7d CGAGGATACCCGAATGTGATGAAGGGCTCAGG(+CAGTGT)GTACCAGTGATGGGGCCACTGGAGACATCTC NGG 10 8 17 8 24 0 100 5 10
    240605160208_yrgg CCAAGGAGATGTCTCCAGTGGCCCCATCACTG(+CAGTGT)GTACCCTGAGCCCTTCATCACATTCGGGTAT NGG 10 8 17 8 24 0 100 5 10
    240605160207_yblk GTCACAGGGCTGGCATCTGGAACACAGCACAG(+CAGTGT)GTACCAAGACAAGGGTAAAATCTTGTAAAGC NGG 10 8 17 8 24 0 100 5 10
    240605160206_dzi1 CTGCTGCTTTACAAGATTTTACCCTTGTCTTG(+CAGTGT)GTACCTGTGCTGTGTTCCAGATGCCAGCCCT NGG 10 8 17 8 24 0 100 5 10
    240605160203_6x28 TTTTGAGACACAGGTTTTCTATGGCCTTCCTG(+CAGTGT)GATCCTCATTGTCTCTCTCCCCAACTCACAG NGG 10 8 17 8 24 0 100 5 10
    240605160202_cpjn CTGTTCTGTGAGTTGGGGAGAGAGACAATGAG(+CAGTGT)GATCCAGGAAGGCCATAGAAAACCTGTGTCT NGG 10 8 17 8 24 0 100 5 10
    240605160159_uo3u AACGGCCATTGGGAAATTTAGGGAAACCCATG(+CAGTGT)GATCCTAGCTGACAGGAGGAGTAGGGTCATT NGG 10 8 17 8 24 0 100 5 10