Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605155930_tf7z AAACAAGCTTAGGGGACTGAGGGGAGCCGGGG(+CAGTGT)GATCCACAGCACCCAAAAGGTGGAATAAGTT NGG 10 8 17 8 24 0 100 5 10
    240605155928_fgz4 AAAGGGGTTTTGATAGAAGGAAGAGACTTCCG(+CAGTGT)GTACCATTGTAACTAAAGTGTCAGTCATTTT NGG 10 8 17 8 24 0 100 5 10
    240605155927_1880 TAGTTAAAATGACTGACACTTTAGTTACAATG(+CAGTGT)GTACCGGAAGTCTCTTCCTTCTATCAAAACC NGG 10 8 17 8 24 0 100 5 10
    240605155926_o3i7 ACATCTCCTGCCTCAGTCTTCAAAGCTATTTG(+CAGTGT)GATCCATAAGTTTGTTATATGCATCTTCTTG NGG 10 8 17 8 24 0 100 5 10
    240605155924_h1vs ACATGCAAGAAGATGCATATAACAAACTTATG(+CAGTGT)GATCCAAATAGCTTTGAAGACTGAGGCAGGA NGG 10 8 17 8 24 0 100 5 10
    240605155923_ogz8 CAAGGTAATGAGTTTCGTGGTAGCATTTTGAG(+CAGTGT)GATCCATGTCATCGTTCATTGTTCTTTTCCT NGG 10 8 17 8 24 0 100 5 10
    240605155922_k63a GGGCTAGGAAAAGAACAATGAACGATGACATG(+CAGTGT)GATCCTCAAAATGCTACCACGAAACTCATTA NGG 10 8 17 8 24 0 100 5 10
    240605155921_zfc8 CTTGCAAAAGCCAAAGCTGCCTGAGTAGTCTG(+CAGTGT)GATCCTGCAACATCACTTTGCTTCAGGAGTT NGG 10 8 17 8 24 0 100 5 10
    240605155919_2ngy AAACTAACTCCTGAAGCAAAGTGATGTTGCAG(+CAGTGT)GATCCAGACTACTCAGGCAGCTTTGGCTTTT NGG 10 8 17 8 24 0 100 5 10
    240605155918_xu87 CTCCAAAGCTAGAGTAATCATGAGGGAGGCAG(+CAGTGT)GATCCACGGAGAATCACAAAGGATGCATTTT NGG 10 8 17 8 24 0 100 5 10