Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605124830_7bez CCTCTGTGGGAACAACAGCCCTGAGGAAACAG(+CAGTGT)GTACCTGAGAAAACCCAGAAAGCCAGCTGAG NGG 10 8 17 8 24 0 100 5 10
    240605124828_mrc4 GGCTGCTGGCCTCCCCTCCATCCTCCAGGACG(+CAGTGT)GATCCATGTTCATTCCTATCCATACCTTGGT NGG 10 8 17 8 24 0 100 5 10
    240605124827_ok79 GTAGGACCAAGGTATGGATAGGAATGAACATG(+CAGTGT)GATCCGTCCTGGAGGATGGAGGGGAGGCCAG NGG 10 8 17 8 24 0 100 5 10
    240605124826_0vaa TATTTGGCCAGCCCTCAACCTCCTCTAGTTTG(+CAGTGT)GATCCAAGGGCCCTGTATGTATGAGGAGTAA NGG 10 8 17 8 24 0 100 5 10
    240605124823_gb0k CTACTTTACTCCTCATACATACAGGGCCCTTG(+CAGTGT)GATCCAAACTAGAGGAGGTTGAGGGCTGGCC NGG 10 8 17 8 24 0 100 5 10
    240605124821_mkls CCAAAAAAAAAAAAGTGCGTGGGAGGAGCCGG(+CAGTGT)GATCCTTTGGTCTCCCTCTCTGCTCCACTTT NGG 10 8 17 8 24 0 100 5 10
    240605124819_55y6 GAGGCAAAGTGGAGCAGAGAGGGAGACCAAAG(+CAGTGT)GATCCCGGCTCCTCCCACGCACTTTTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240605124818_isud CCTGAGCCTCCCTCGGGCACCTGTTGCTTCTG(+CAGTGT)GATCCGAATAATCCAGGAGGCTCCCCACCCT NGG 10 8 17 8 24 0 100 5 10
    240605124816_2pqa GCCACAGGGTGGGGAGCCTCCTGGATTATTCG(+CAGTGT)GATCCAGAAGCAACAGGTGCCCGAGGGAGGC NGG 10 8 17 8 24 0 100 5 10
    240605124815_xj16 GCCACACACATACCGAGACTCTGTGAATGATG(+CAGTGT)GTACCACTCTACCTAGGACAGGAGAGACCGA NGG 10 8 17 8 24 0 100 5 10