Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605124758_7qti ATGACTCAAGCTCACCAGCAGGGGGCCTCCTG(+CAGTGT)GTACCTCCTTTGCTCTTGGGCCTGCTTTTAC NGG 10 8 17 8 24 0 100 5 10
    240605124756_1tvk CACCAGTGTTCGGGTGGAAGCATAGGAAACAG(+CAGTGT)GATCCCGCAGTGGCAAGGTCAGGCCACCTGC NGG 10 8 17 8 24 0 100 5 10
    240605124755_426n CCCAGGCAGGTGGCCTGACCTTGCCACTGCGG(+CAGTGT)GATCCTGTTTCCTATGCTTCCACCCGAACAC NGG 10 8 17 8 24 0 100 5 10
    240605124754_72bi TCCAAAATGTGAATAACTTGTCAGAGCAGCTG(+CAGTGT)GTACCCCTGATCGGCTGGGTCTGCTTCCGGT NGG 10 8 17 8 24 0 100 5 10
    240605124752_qfqr TAGCAACCGGAAGCAGACCCAGCCGATCAGGG(+CAGTGT)GTACCAGCTGCTCTGACAAGTTATTCACATT NGG 10 8 17 8 24 0 100 5 10
    240605124751_7hde ACGAGGTGGCTGGAGGGTGCTCTCAGCTGCAG(+CAGTGT)GTACCAGACCCAGGACAGGAAATGTCAGCCA NGG 10 8 17 8 24 0 100 5 10
    240605124749_jux2 GCTGATGGCTGACATTTCCTGTCCTGGGTCTG(+CAGTGT)GTACCTGCAGCTGAGAGCACCCTCCAGCCAC NGG 10 8 17 8 24 0 100 5 10
    240605124747_sr7c GCCTCTCCAGCCCCCGCTGGGCTGCAGCCAGG(+CAGTGT)GTACCATCAACAGCCATGTTAAGGTTCTGAT NGG 10 8 17 8 24 0 100 5 10
    240605124746_hgfd TTTTCATCAGAACCTTAACATGGCTGTTGATG(+CAGTGT)GTACCCTGGCTGCAGCCCAGCGGGGGCTGGA NGG 10 8 17 8 24 0 100 5 10
    240605124744_h8vz CTTCTCCATGGCTGCCAGGCCCCCCCAGGCAG(+CAGTGT)GTACCTGCAGCGGCTGCCAGCCTGAGGGACT NGG 10 8 17 8 24 0 100 5 10