Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605124655_l76r CTTGGTTTCACCATCCCCGTCCTTTCCCCACG(+CAGTGT)GATCCACCAGGATTGAAGGAGCTTCGGACCC NGG 10 8 17 8 24 0 100 5 10
    240605124653_jwng AGGGTCTTATGTAGCCTCACTATGTAGCCAAG(+CAGTGT)GATCCCCTGCCTCCACCTCCCGAGTACAGGG NGG 10 8 17 8 24 0 100 5 10
    240605124651_muxc GTGAGCCCTGTACTCGGGAGGTGGAGGCAGGG(+CAGTGT)GATCCTTGGCTACATAGTGAGGCTACATAAG NGG 10 8 17 8 24 0 100 5 10
    240605124650_vcq3 TACAAGGACACAGATGGTTTGTATTGTCATCG(+CAGTGT)GATCCAGTTGTCATGGACCCATGCCTGGGGA NGG 10 8 17 8 24 0 100 5 10
    240605124649_pqm0 AGATCTCCCCAGGCATGGGTCCATGACAACTG(+CAGTGT)GATCCGATGACAATACAAACCATCTGTGTCC NGG 10 8 17 8 24 0 100 5 10
    240605124647_sz5d ATCTGATGCCCCTTGCGCTCTCTGAGGGTTGG(+CAGTGT)GTACCATTTTCAATTTCAGAAGAGGACAGAG NGG 10 8 17 8 24 0 100 5 10
    240605124646_mc83 ATAATCTCTGTCCTCTTCTGAAATTGAAAATG(+CAGTGT)GTACCCAACCCTCAGAGAGCGCAAGGGGCAT NGG 10 8 17 8 24 0 100 5 10
    240605124643_kbrr TGTGGACAGTGGGCAGAAGCAGAGGCTCCCTG(+CAGTGT)GATCCTGAACAAGGGCCCCAAAAGCAAGTTC NGG 10 8 17 8 24 0 100 5 10
    240605124642_je4v CTTTAGAACTTGCTTTTGGGGCCCTTGTTCAG(+CAGTGT)GATCCAGGGAGCCTCTGCTTCTGCCCACTGT NGG 10 8 17 8 24 0 100 5 10
    240605124639_gz35 CGTGCCATCCCCATCAGGACCTCAGACCTGGG(+CAGTGT)GTACCTGAACTCTGTCCCTCTGCTGCCGCCT NGG 10 8 17 8 24 0 100 5 10