Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605124607_le58 TTGGCCAAAGAGCAGCTAGTATCTACCACATG(+CAGTGT)GATCCTTACAGGGGCAAATTCTCAGTTTGTT NGG 10 8 17 8 24 0 100 5 10
    240605124606_0c3x TTAAAATCTAATACCTGTAGCACAAAGTTGTG(+CAGTGT)GATCCATATAGCAGAAATTGGCTGCCCTTTG NGG 10 8 17 8 24 0 100 5 10
    240605124605_06pv GGGGTCAAAGGGCAGCCAATTTCTGCTATATG(+CAGTGT)GATCCACAACTTTGTGCTACAGGTATTAGAT NGG 10 8 17 8 24 0 100 5 10
    240605124603_hz33 CCACATGGTGGTTCACAACCATCTGTAATGGG(+CAGTGT)GATCCATATACATAAATAAACCTTTTAAAAA NGG 10 8 17 8 24 0 100 5 10
    240605124602_c2is AATATTTTTTAAAAGGTTTATTTATGTATATG(+CAGTGT)GATCCCCATTACAGATGGTTGTGAACCACCA NGG 10 8 17 8 24 0 100 5 10
    240605124601_1k2t TTAAAATCTAATACCTGTAGCACAAAGTTGTG(+CAGTGT)GATCCATATAGCAGAAATTGGCTGCCCTTTG NGG 10 8 17 8 24 0 100 5 10
    240605124559_h8tj GGGGTCAAAGGGCAGCCAATTTCTGCTATATG(+CAGTGT)GATCCACAACTTTGTGCTACAGGTATTAGAT NGG 10 8 17 8 24 0 100 5 10
    240605124557_6b0f CTAGGGAGCCATCTGGGATGTTGGCATGTTGG(+CAGTGT)GTACCTGTCCTGTGACACTTGATTGTGCTTG NGG 10 8 17 8 24 0 100 5 10
    240605124556_xvxc CCACCCAAGCACAATCAAGTGTCACAGGACAG(+CAGTGT)GTACCCAACATGCCAACATCCCAGATGGCTC NGG 10 8 17 8 24 0 100 5 10
    240605124555_a3rd GGAGAGTCACTGGCCTCTCCCGTTGGTTACAG(+CAGTGT)GTACCCTTATACCCTGGATGTTAGCGTTGTA NGG 10 8 17 8 24 0 100 5 10