Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605124553_ifrj ACAACTACAACGCTAACATCCAGGGTATAAGG(+CAGTGT)GTACCTGTAACCAACGGGAGAGGCCAGTGAC NGG 10 8 17 8 24 0 100 5 10
    240605124551_3ghy CTTGAAAAGCTGGCAGCACTGCAGCCTCCCTG(+CAGTGT)GTACCATTGGTCTGACAGACACAGTTCCAGA NGG 10 8 17 8 24 0 100 5 10
    240605124549_74ua GACTATCTGGAACTGTGTCTGTCAGACCAATG(+CAGTGT)GTACCAGGGAGGCTGCAGTGCTGCCAGCTTT NGG 10 8 17 8 24 0 100 5 10
    240605124547_rnca CGGAGGATGAAGCCCATCCCTTGCAGATGGGG(+CAGTGT)GTACCCATGCTTTGATGATGTTCACAGAGGG NGG 10 8 17 8 24 0 100 5 10
    240605124545_4b4l AGAAGCCCTCTGTGAACATCATCAAAGCATGG(+CAGTGT)GTACCCCCATCTGCAAGGGATGGGCTTCATC NGG 10 8 17 8 24 0 100 5 10
    240605124543_lrve TCGTGTCTATCCAGGCTGGGGGCCAGACTGGG(+CAGTGT)GATCCCTTTCTGGGGTCTGCACTGTGTGTGT NGG 10 8 17 8 24 0 100 5 10
    240605124542_xpkn CACACACACACACAGTGCAGACCCCAGAAAGG(+CAGTGT)GATCCCCAGTCTGGCCCCCAGCCTGGATAGA NGG 10 8 17 8 24 0 100 5 10
    240605124540_8t0s TGACTTTTCATGGGCGTGCTCTAGGAATCCTG(+CAGTGT)GATCCCACTAAACTTCCATGGCTGGTTGGGC NGG 10 8 17 8 24 0 100 5 10
    240605124538_eqvg CCCGTGCCCAACCAGCCATGGAAGTTTAGTGG(+CAGTGT)GATCCAGGATTCCTAGAGCACGCCCATGAAA NGG 10 8 17 8 24 0 100 5 10
    240605124535_b8vy GCCCAGCCTGACTGCTGTGCTCTGGGCCTGGG(+CAGTGT)GATCCATGAGGTTCTTATGTAGAAGCTGAGG NGG 10 8 17 8 24 0 100 5 10