Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605124500_pp5p CTAGCCTATCCCTAACTTCCCCACCATGGCAG(+CAGTGT)GTACCGATCCTAGAGTCCCAAGTGGTAGAGC NGG 10 8 17 8 24 0 100 5 10
    240605124459_sx9c CTTAGTCCTTAGACTTGATGTGCCATGGATTG(+CAGTGT)GTACCATGGGTGGTAGTGGGGGTGGTGACAG NGG 10 8 17 8 24 0 100 5 10
    240605124456_mt2c GAGCCCTGTCACCACCCCCACTACCACCCATG(+CAGTGT)GTACCAATCCATGGCACATCAAGTCTAAGGA NGG 10 8 17 8 24 0 100 5 10
    240605124454_zi0k CAGTCAGGGACAAGGCTGTCTTCAGGTGTGGG(+CAGTGT)GATCCTGGGACTCCAACATGCAGGAAGGGGC NGG 10 8 17 8 24 0 100 5 10
    240605124452_xny5 GTAGAGCCCCTTCCTGCATGTTGGAGTCCCAG(+CAGTGT)GATCCCCACACCTGAAGACAGCCTTGTCCCT NGG 10 8 17 8 24 0 100 5 10
    240605124450_6ru9 GCTCACAATGGCCTACAACTCCAGTTCCTGAG(+CAGTGT)GATCCAGTACCCTTACATTCACACACATGAA NGG 10 8 17 8 24 0 100 5 10
    240605124449_pek1 TTGCCTTCATGTGTGTGAATGTAAGGGTACTG(+CAGTGT)GATCCTCAGGAACTGGAGTTGTAGGCCATTG NGG 10 8 17 8 24 0 100 5 10
    240605124447_8jwk CTTATCTTCAGACACACTAGAAGAGGCCATCG(+CAGTGT)GATCCTATTACAGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240605124446_saik ATCACATGGTGGCTCACAACCATCTGTAATAG(+CAGTGT)GATCCGATGGCCTCTTCTAGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240605124443_w5bu CCCTTCCCACTGAGATGCCTGGGCAGCCATGG(+CAGTGT)GTACCCCGAGGCTGATAACACATCAGAGGTG NGG 10 8 17 8 24 0 100 5 10