Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605124534_kqen AGAGACCTCAGCTTCTACATAAGAACCTCATG(+CAGTGT)GATCCCCAGGCCCAGAGCACAGCAGTCAGGC NGG 10 8 17 8 24 0 100 5 10
    240605124533_88ft CCTCTTCCCTCTGCCCAGCACCTAAGAGCAGG(+CAGTGT)GATCCCACGGCGGGGAGATGAGCAGGGGTGC NGG 10 8 17 8 24 0 100 5 10
    240605124530_06xb AGACCGCACCCCTGCTCATCTCCCCGCCGTGG(+CAGTGT)GATCCCTGCTCTTAGGTGCTGGGCAGAGGGA NGG 10 8 17 8 24 0 100 5 10
    240605124529_az4e TCCTAAGGGAGCCAGATGTCAAGTCCAGGGTG(+CAGTGT)GTACCCGATAACAATCTCCTGGGCTTCAGGT NGG 10 8 17 8 24 0 100 5 10
    240605124527_9ri4 TCAGGACCTGAAGCCCAGGAGATTGTTATCGG(+CAGTGT)GTACCACCCTGGACTTGACATCTGGCTCCCT NGG 10 8 17 8 24 0 100 5 10
    240605124525_co7b GTCAACTAAGGCGGCTATTGTTTGTGCCTCAG(+CAGTGT)GATCCTAAGGGAGCCAGATGTCAAGTCCAGG NGG 10 8 17 8 24 0 100 5 10
    240605124523_eugo ACCACCCTGGACTTGACATCTGGCTCCCTTAG(+CAGTGT)GATCCTGAGGCACAAACAATAGCCGCCTTAG NGG 10 8 17 8 24 0 100 5 10
    240605124522_58c2 TCCAGCTTCTGCCAAGCTCCCCTAAGGAATTG(+CAGTGT)GATCCAAAGTAAAATGGCCCATAGCTCTCCT NGG 10 8 17 8 24 0 100 5 10
    240605124521_kdut TGACCAGGAGAGCTATGGGCCATTTTACTTTG(+CAGTGT)GATCCAATTCCTTAGGGGAGCTTGGCAGAAG NGG 10 8 17 8 24 0 100 5 10
    240605124518_54mp GAGGAGGTGTAGACTGGGAACTAGTGCCTGGG(+CAGTGT)GTACCCCATCTTGAGGTCAGCAGCAAGTGGG NGG 10 8 17 8 24 0 100 5 10